Filters

5 MethylCytosine Antibody, FITC Conjugated

5 MethylCytosine Antibody, FITC Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name FITC Conjugated, 5 MethylCytosine Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target Antigen5 MethylCytosine
SpecificityThis is a highly specific antibody against 5 MethylCytosine
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated 5 MethylCytosine
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
CrossreactivityOthers
Crossreactive species details5 MethylCytosine
Antigen background DNA is methylated on cytidines by DNA methylases (DNMTs)to generate 5-methylcytidine (5-mC), Several enzymes, The reverse reaction (cytidine demethylation) starts with its oxidation to hydroxymethyl- (5-hmC), a potent epigenetics marker and regulator of gene expression, and carboxy- (5-caC) cytidine, formyl- (5-fC), including the Tet family of proteins have been implicated in cytidine demethylation, Cytidine is a nucleoside formed by a cytosine attached to a ribose ring via a beta-N1-glycosidic bond
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms 5 Me Cytidine, 5 MeCyd, 5 Methyl Cytidine, 5 MethylCytosine, 5 m C, 5 mC, 5 me C, 5-Me Cytidine, 5-Methyl-Cytosine, 5-MethylCytidine, 5-mC, 5-Methyl Cytidine
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene target5 MethylCytosine Conjugated
Short name FITC Conjugated, 5 MethylCytosine Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, 5 MethylCytosine (Antibody to)
Alternative techniqueantibodies

Subscribe to our Newsletter