| Antigen detectable with this ELISA kit | A Disintegrin And Metalloprotease 12 (ADAM12) |
| Species reactivity | Homo sapiens (Human) |
| Range of detection | 1250pg/mL, 156, 2500pg/mL, 312, 625pg/mL, 78, 125-5000pg/mL The standard curve concentrations used for the ELISA&rsquo, 125pg/mL, 25pg/mL, 5pg/mL, 78, s were 5000pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 24pg/mL |
| Application | ELISA |
| Sample types tested for this ELISA kit | plasma and other biological fluids, Serum |
| Time for performing t hourse test | 4, 5 hoursours |
| Test principle | 10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 12 (ADAM12), The concentration of A Disintegrin And Metalloprotease 12 (ADAM12) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 12 (ADAM12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain A Disintegrin And Metalloprotease 12 (ADAM12), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling | Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory | Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties | E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test | ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target | A Disintegrin Metalloprotease 12 (ADAM12) |
| Short name | A Disintegrin And Metalloprotease 12 (ADAM12) ELISA kit |
| Technique | &hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host | antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name | A Disintegrin And Metalloprotease 12 (ADAM metallopeptidase domain 12) Enzyme-linked immunosorbent assay reagent |
| Alternative technique | elisas |
| Alternative to gene target | ADAM12 and IDBG-93032 and ENSG00000148848 and 8038, ADAM12-OT1 and CAR10 and MCMP and MCMPMltna and MLTN and MLTNA, Adam12 and IDBG-211287 and ENSMUSG00000054555 and 11489, BT, Extracellular, SH3 domain binding, this GO :0004222 and metalloendopeptidase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006508 and proteolysis and biological process this GO :0007155 and cell adhesion and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007520 and myoblast fusion and biological process this GO :0008237 and metallopeptidase activity and molecular function this GO :0008270 and zinc ion binding and molecular function this GO :0016021 and integral component of membrane and cellular component this GO :0017124 and SH3 domain binding and molecular function, this GO :0004222: metalloendopeptidase activity, this GO :0004222: metalloendopeptidase activity and also this GO :0005515: protein binding and also this GO :0008237: metallopeptidase activity and also this GO :0008270: zinc ion binding and also this GO :0017124: SH3 domain binding, this GO :0005515: protein binding, this GO :0008237: metallopeptidase activity, this GO :0008270: zinc ion binding, this GO :0017124: SH3 domain binding, 29890 and IDBG-640937 and ENSBTAG00000012444 and 407229, ADAM metallopeptidase domain 12 |
| Identity | 190 |
| Gene | ADAM12 |
| Long gene name | ADAM metallopeptidase domain 12 |
| Synonyms gene name | a disintegrin and metalloproteinase domain 12 (meltrin alpha) |
| Synonyms | MCMPMltna MLTN |
| Synonyms name | meltrin alpha |
| Locus | 10q26, 2 |
| Discovery year | 1998-12-01 |
| GenBank acession | AF023476 |
| Entrez gene record | 8038 |
| Pubmed identfication | 9417060 18342566 |
| Classification | ADAM metallopeptidase domain containing |
| Havana BLAST/BLAT | OTTHUMG00000019243 |