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A Raf (Ser214) Antibody, Biotin Conjugated

A Raf (Ser214) Antibody, Biotin Conjugated size: 0.1ml 350

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 350
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name Biotin Conjugated, A Raf (Ser214) Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationBiotinylated
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenA Raf Ser214
SpecificityThis is a highly specific antibody against A Raf Ser214
ModificationPhosphorylation
Modification siteSer214
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic phosphopeptide derived from human A Raf around the phosphorylation site of Ser214
Gene ID number369
Tested applications IHC-P, WB
Recommended dilutions IHC-P(1:100-500), WB(1:100-1000)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background 14-3-3 is also known to be an activator of RAFs, A RAF is expressed at a very high concentration in urogenital and kidney tissues, Activated RAF directly phosphorylates and activates MEK, RAF 1 and B RAF, RAF 1 is known to be downstream of Ras and binds to it by an N terminal noncatalytic domain, These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway, This domain is conserved in A RAF and B RAF, The RAF family of signaling proteins has three isoforms: A RAF
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation EmissionN/A
Synonyms A Raf proto oncogene serine/threonine protein kinase, ARAF, ARAF 1, ARAF1, ARAF_HUMAN, ARaf proto oncogene serine/threonine protein kinase, Oncogene Araf1, Oncogene PKS2, PKS, PKS 2, PKS2, Proto oncogene Pks, RAFA 1, RAFA1, Ras binding protein DA Raf, v raf murine sarcoma 3611 viral oncogene homolog, v raf murine sarcoma 3611 viral oncogene homolog 1, v raf oncogene homolog 1 murine sarcoma 3611 virus, A raf 1
Properties Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetA Raf (Ser214) Conjugated
Short name Conjugated, A Raf (Ser214) Antibody
Technique antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelBiotin
Alternative name biotinilated coupled, A Raf (Ser214) (Antibody to)
Alternative techniqueantibodies

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