Name : Actin Antibody / ACTA1
Supplier : NJS POLY
Price :406
SKU : GEN1927061599
| Category | Antibody |
| Concentration | 2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form | Antigen affinity purified |
| Conjugation | Unconjugated |
| Clone | Polyclonal antibody |
| Recognised antigen | Actin / ACTA1 |
| Host animal | Rabbit (Oryctolagus cuniculus) |
| Clonality | Polyclonal (rabbit origin) |
| Species reactivity | Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications | IHC-P, WB |
| Recommended dilutions | 1-0, 5-1ug/ml, 5ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes | Optimal dilution of the Actin antibody should be determined by the researcher |
| Intented use | This Actin antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot # | P68133 |
| Purity | Antigen affinity |
| Description | It varies in amount, The actins exhibit over 90% sequence homology, The isoforms are comprised of three alpha-actin, a highly conserved protein, being related to the type of differentiation and to the functional state of cells and tissues, but each isoform has a unique NH2-terminal sequence, is a major component of both the cytoskeletal and contractile structures in the cell types, one beta-actin, two gamma-actin, Actin |
| Immunogen | Amino acids HETTYNSIMKCDIDIRKDLYANNVMSGGTTMY of human Actin were used as the immunogen for the Actin antibody |
| Storage | Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Actin antibody may be kept for up to one month refrigerated at +4 degrees C, After reconstitution, For long-term |
| Localization | Cytoplasmic |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target | Actin / ACTA1 |
| Short name | Actin Antibody / ACTA1 |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Alternative name | Actin (Antibody to) / ACTA1 |
| Alternative technique | antibodies |
| Identity | 129 |
| Gene | ACTA1 |
| Long gene name | actin, alpha 1, skeletal muscle |
| Synonyms gene | ACTA |
| Synonyms | NEM3 |
| Synonyms name | nemaline myopathy type 3 |
| Locus | 1q42, 13 |
| Discovery year | 1986-01-01 |
| GenBank acession | J00068 |
| Entrez gene record | 58 |
| Pubmed identfication | 10072583 6865942 |
| RefSeq identity | NM_001100 |
| Classification | Actins |
| Havana BLAST/BLAT | OTTHUMG00000038006 |
| Locus Specific Databases | Leiden Muscular Dystrophy pages LRG_429 |