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Adenovirus 5 E1A Antibody, FITC Conjugated

Adenovirus 5 E1A Antibody, FITC Conjugated size: 0.1ml 333

Price 333
Size 0.1ml
Long name FITC Conjugated, Adenovirus 5 E1A Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenAdenovirus 5 E1A
SpecificityThis is a highly specific antibody against Adenovirus 5 E1A
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
Immunogen_range180-230/289
Subcellular locationNucleus
SourceKLH conjugated synthetic peptide derived from human Adenovirus 5 E1A
Swiss ProtP03255
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
CrossreactivityVirus
Crossreactive species detailsAdenovirus5
Antigen background E1A forms specific complexes with cellular proteins including p105 causing inhibition of the cell cycle inducing arresting function of p105, E1A gene products are necessary for normal levels of transcription of the other early regions of the adenovirus genome during productive infection and are able to either activate or repress the transcription of specific cellular genes, E1A is sufficient for partial transformation and immortalization of primary cells, is localized within the left most 11% of the viral genome and consists of two transcriptional units E1A and E1B, responsible for transforming activity, The early region (E1) of the adenovirus genome
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Synonyms Control protein E1A, E1A, E1A_ADE05, Early E1A protein, Human adenovirus C, Human adenovirus type 5, pfam02703, Adenovirus early E1A protein
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetAdenovirus 5 E1A Conjugated
Short name FITC Conjugated, Adenovirus 5 E1A Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, Adenovirus 5 E1A (Antibody to)
Alternative techniqueantibodies

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