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ALAS1 Antibody, FITC Conjugated

ALAS1 Antibody, FITC Conjugated size: 0.1ml 333

Supplier BIOSS PRIMARY CONJUGATED ANTIBODIES 1
Price 333
Size 0.1ml
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Long name FITC Conjugated, ALAS1 Polyclonal Antibody
CategoryConjugated Primary Antibodies
ConjugationAnti-FITC Antibody
Host OrganismRabbit (Oryctolagus cuniculus)
Target AntigenALAS1
SpecificityThis is a highly specific antibody against ALAS1
ModificationUnmodified
Modification siteNone
ClonalityPolyclonal
ClonePolyclonal antibody
Concentration1ug per 1ul
SourceKLH conjugated synthetic peptide derived from human ALAS1/ALAS-H
Gene ID number211
Tested applicationsIF(IHC-P)
Recommended dilutionsIF(IHC-P)(1:50-200)
Crossreactivity Mouse, Rat, Human
Crossreactive species details not every possible cross-reactivity is known, Due to limited amount of testing and knowledge
Antigen background ALAS-E, ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells, NF-E2 and EKLF, Since the ALAS gene maps to the X chromosome, Succinyl CoA associates with ALAS-E in protein conformation change and translocation of ALAS-E into the mitochondria and does not interact with ALAS-H, The ALAS-E 5'-flanking region contains binding sites for nuclear activators such as GATA-1, The erythroid-specific isoenzyme, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals, mutation of the gene leads to the pyridoxine-refractory X-linked sideroblastic anemia, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver, 5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS
Purification methodThis antibody was purified via Protein A
Storage conditions 50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA
Excitation Emission494nm/518nm
Properties Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°
Gene targetALAS1 Conjugated
Short name FITC Conjugated, ALAS1 Antibody
Technique Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody
LabelFITC
Alternative name fluorecein coupled, ALAS1 (Antibody to)
Alternative techniqueantibodies
Identity 396
Gene ALAS1
Long gene name 5', -aminolevulinate synthase 1
Synonyms gene ALAS3 ALAS
Synonyms gene name aminolevulinate, delta-, synthase 1
Locus 3p21, 2
Discovery year 1986-01-01
GenBank acession X56351
Entrez gene record 211
Havana BLAST/BLAT OTTHUMG00000158108

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