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Anit-GAPDH Antibody

Anit-GAPDH Antibody size: 0.4 ml 406

Supplier NJS POLY
Price 406
Size 0.4 ml
CategoryAntibody
Concentration pH 7, with 0, 09% sodium azide, 4, In 1X PBS
FormPurified
ConjugationUnconjugated
ClonePolyclonal antibody
Recognised antigenAnit-GAPDH
Host animalRabbit (Oryctolagus cuniculus)
ClonalityPolyclonal (rabbit origin)
Species reactivity Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens)
Tested applications ELISA, IHC, WB, IF
Recommended dilutionsIHC (Paraffin): 1:10-1:50, Immunofluorescence: 1:10-1:50, Western blot: 1:500-1000
Notes Titration of the anti-GAPDH antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts
Intented useThis anti-GAPDH antibodyis to be used only for research purposes and not for diagnostics
Uniprot #P04406
PurityPurified
Description The enzyme exists as a tetramer of identical chains, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism
ImmunogenA portion of amino acids 62-91 from the human protein was used as the immunogen for this anti-GAPDH antibody
Storage Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the anti-GAPDH antibody and store frozen at -20 deg
PropertiesC, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°
Gene targetAnit-GAPDH
Short nameAnit-GAPDH Antibody
Technique antibodies against human proteins, antibodies for, Antibody
Alternative nameAnit-GAPDH (Antibody to)
Alternative techniqueantibodies
Identity 4141
Gene GAPDH
Long gene name glyceraldehyde-3-phosphate dehydrogenase
Synonyms gene GAPD
Locus 12p13, 31
Discovery year 2001-06-22
GenBank acession AF261085
Entrez gene record 2597
Pubmed identfication 3170585
RefSeq identity NM_002046
Havana BLAST/BLAT OTTHUMG00000137379

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