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Anti-AMPK beta 1 + 2 (Polyclonal), ALEXA Fluor 594

Anti-AMPK beta 1 + 2 (Polyclonal), ALEXA Fluor 594 size: 100 microliters 489

Price 489
Size 100 microliters
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideAMPK beta 1 + 2
SpecificityThis antibody reacts specifically with AMPK beta 1 + 2
ModificationNo modification has been applied to this antibody
Modification siteNone
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locations Nucleus, Cytoplasm
Antigen SourceKLH conjugated synthetic peptide derived from human AMPK beta 1
Gene ID5564
Swiss ProtQ9Y478
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, AMPK acts via direct phosphorylation of metabolic enzymes, Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, In response to reduction of intracellular ATP levels, PRKAG2 or PRKAG3), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism, and by longer-term effects via phosphorylation of transcription regulators, as well as cell growth and proliferation, carbohydrate and lipid biosynthesis, probably by indirectly activating myosin, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, Non-catalytic subunit of AMP-activated protein kinase (AMPK)
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Synonyms AMPK AMPKa2
Also known asAMPK beta 1 + 2 Polyclonal Antibody
Other nameAnti-AMPK beta 1 + 2 Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetAMPK beta 1 + 2
Short nameAnti-AMPK beta 1 + 2 (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-AMPK b 1 and 2 (polyclonal)
Alternative techniquepolyclonals
Identity 9377
Gene PRKAA2
Long gene name protein kinase AMP-activated catalytic subunit alpha 2
Synonyms gene PRKAA
Synonyms gene name AMP-activated, alpha 2 catalytic subunit , protein kinase
Locus 1p32, 2
Discovery year 1995-12-12
GenBank acession BC069823
Entrez gene record 5563
Pubmed identfication 7959015
RefSeq identity NM_006252
Havana BLAST/BLAT OTTHUMG00000008282

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