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Anti-APC10 (Polyclonal), ALEXA Fluor 594

Anti-APC10 (Polyclonal), ALEXA Fluor 594 size: 100 microliters 489

Supplier BIOSS POLYCLONAL ANTIBODIES
Price 489
Size 100 microliters
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideAPC10
SpecificityThis antibody reacts specifically with APC10
ModificationNo modification has been applied to this antibody
Modification siteNone
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsN/A
Antigen SourceKLH conjugated synthetic peptide derived from human APC10
Gene ID10393
Swiss ProtN/A
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information APC, APC is phosphorylated, APC is under tight control by a number of regulatory factors, APC10 also localizes to kinetochores from prophase to anaphase, APC10 binds to core APC subunits throughout the cell cycle, APC10 binds to the C-terminus of CDC27/APC3, APC10 contains a Doc1 homology domain, APC10 is localized in centrosomes and mitotic spindles, CDC20 and CDH1 directly bind to and activate the cyclin-ubiquitination activity of APCs, CDH1 and MAD2, During mitosis, In contrast, MAD2 inhibits APC by forming a ternary complex with CDC20 and APC, Specifically, Specifically, accomplishes this progression through the ubiquitination of mitotic cyclins and other regulatory proteins that are targeted for destruction during cell division, and thus activated, and to the midbody in telophase and cytokinesis, by protein kinases Cdk1/cyclin B and polo-like kinase (Plk), including CDC20, or cyclosome, the anaphase-promoting complex (APC) acts in a cell-cycle dependent manner to promote the separation of sister chromatids during the transition between metaphase and anaphase in mitosis, thus preventing APC activation, which is a beta-sandwich structure common to many other putative E3 ubiquitin ligases, Composed of more than ten subunits
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Synonyms APC10 DOC1 DKFZP564L0562
Also known asAPC10 Polyclonal Antibody
Other nameAnti-APC10 Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetAPC10
Short nameAnti-APC10 (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-APC10 (polyclonal)
Alternative techniquepolyclonals
Identity 24077
Gene ANAPC10
Long gene name anaphase promoting complex subunit 10
Locus 4q31, 21
Discovery year 2004-05-21
GenBank acession AF132794
Entrez gene record 10393
Pubmed identfication 10318877 11230166
RefSeq identity NM_014885
Classification Anaphase promoting complex
Havana BLAST/BLAT OTTHUMG00000161477

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