Filters

Anti-ATF2 (Ser44) (Polyclonal), ALEXA Fluor 594

Anti-ATF2 (Ser44) (Polyclonal), ALEXA Fluor 594 size: 100 microliters 516

Supplier BIOSS POLYCLONAL ANTIBODIES
Price 516
Size 100 microliters
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideATF2 Ser44
SpecificityThis antibody reacts specifically with ATF2 (Ser44)
ModificationPhosphorylation
Modification siteSer44
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsN/A
Antigen SourceKLH conjugated synthetic phosphopeptide derived from mouse ATF2 around the phosphorylation site of Ser44
Gene ID11909
Swiss ProtN/A
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information ATF2 is a nuclear protein that binds DNA as a dimer and can form dimers with members of the ATF/CREB and Jun/Fos families, ATF2 is abundantly expressed in brain, Following dimerization ATF2 becomes a short lived protein that undergoes ubiquitination and proteolysis, It is a stronger activator as a heterodimer with cJun than as a homodimer, Many of these genes are important in cell growth and differentiation, Several isoforms of ATF2 arise by differential splicing, Stimulation of the transcriptional activity of ATF2 occurs following cellular stress induced by several genotoxic agents, The stable native full length ATF2 is transcriptionally inactive as a result of an inhibitory direct intramolecular interaction of its carboxy terminal DNA binding domain with the amino terminal transactivation domain, This activation requires phosphorylation of two threonine residues in ATF2 by both JNK/SAP kinase and p38 MAP kinase, and UV irradiation, and in stress and immune responses, inflammatory cytokines, seemingly in a protein phosphatase-dependent mechanism, ATF2 is a member of the ATF/CREB family of basic region leucine zipper DNA binding proteins that regulates transcription by binding to a consensus cAMP response element (CRE) in the promoter of various viral and cellular genes
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Synonyms ATF1 ATF2 HFB1-GDNF
Also known asATF2 (Ser44) Polyclonal Antibody
Other nameAnti-ATF2 (Ser44) Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetATF2 (Ser44)
Short nameAnti-ATF2 (Ser44) (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-ATF2 (Ser44) (polyclonal)
Alternative techniquepolyclonals
Identity 4232
Gene GDNF
Long gene name glial cell derived neurotrophic factor
Synonyms name astrocyte-derived trophic factor glial cell line derived neurotrophic factor glial derived neurotrophic factor
Locus 5p13, 2
Discovery year 1995-05-04
Entrez gene record 2668
Pubmed identfication 8493557
RefSeq identity NM_000514
Classification Endogenous ligands
Havana BLAST/BLAT OTTHUMG00000090809

Similar products

Subscribe to our Newsletter