Filters

Anti-ATP5E (Polyclonal), ALEXA Fluor 594

Anti-ATP5E (Polyclonal), ALEXA Fluor 594 size: 100 microliters 489

Price 489
Size 100 microliters
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideATP5E
SpecificityThis antibody reacts specifically with ATP5E
ModificationNo modification has been applied to this antibody
Modification siteNone
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsN/A
Antigen SourceKLH conjugated synthetic peptide derived from human ATP5E
Gene ID514
Swiss ProtN/A
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive speciesHuman (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information ATPases contain two linked complexes: F1, F0 consists of three subunits: a, F1 consists of three Alpha chains and three Beta chains, Mitochondrial ATPase e chain (ATP5E) localizes to the mitochondria and catalyzes ATP synthesis, The e chain of F1 contains 50 amino acids and is the smallest of the five ATPase F1 chains, and F0, as well as one Gamma chain, b and c, one Delta chain and one e chain, the hydrophilic catalytic core, the membrane-embedded protein channel, which are weakly homologous, Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Also known asATP5E Polyclonal Antibody
Other nameAnti-ATP5E Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetATP5E
Short nameAnti-ATP5E (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-ATP5E (polyclonal)
Alternative techniquepolyclonals
Identity 838
Gene ATP5E
Long gene name ATP synthase, H+ transporting, epsilon subunit , mitochondrial F1 complex
Locus 20q13, 32
Discovery year 1993-02-25
GenBank acession AF077045
Entrez gene record 514
Pubmed identfication 10727396
RefSeq identity NM_001001977
Classification F-type ATPases Mitochondrial complex V: ATP synthase subunits
Havana BLAST/BLAT OTTHUMG00000032854

Subscribe to our Newsletter