Filters

Anti-Btk (Ser180) (Polyclonal), ALEXA Fluor 594

Anti-Btk (Ser180) (Polyclonal), ALEXA Fluor 594 size: 100 microliters 516

Supplier BIOSS POLYCLONAL ANTIBODIES
Price 516
Size 100 microliters
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideBtk Ser180
SpecificityBtk (Ser180), This phosphorylation site is homologous in the listed cross reactive species at the specified location
ModificationPhosphorylation
Modification siteSer180
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locations Nucleus, Cytoplasm
Antigen SourceKLH conjugated synthetic phosphopeptide derived from human Btk around the phosphorylation site of Ser180 [GS(p-S)HR]
Gene ID695
Swiss ProtQ06187
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information ARID3A and NFAT are other transcriptional target of BTK, After BCR engagement and activation at the plasma membrane, BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways, BTK has a dual role in the regulation of apoptosis, BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B, BTK is required for the formation of functional ARID3A DNA-binding complexes, BTK plays also a critical role in transcription regulation, Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation, Especially, GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression, Induces the activity of NF-kappa-B, PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK, Plays an important role in the function of immune cells of innate as well as adaptive immunity, The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense, There is however no evidence that BTK itself binds directly to DNA, Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR, Within the TLR pathway, as a component of the Toll-like receptors (TLR) pathway, differentiation and signaling, followed by activation of the protein kinase C (PKC) family members, igniting the downstream signaling pathway through calcium mobilization, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation, is a critical molecule in regulating TLR9 activation in splenic B-cells, phosphorylates PLCG2 at several sites, which is involved in regulating the expression of hundreds of genes, Non-receptor tyrosine kinase indispensable for B lymphocyte development
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Synonyms AGMX1, ATK, Agammaglobulinemia tyrosine kinase, B-cell progenitor kinase, BPK, BTK, Bruton tyrosine kinase, IMD1, PSCTK1, Tyrosine-protein kinase BTK, XLA, AT
Also known asBtk (Ser180) Polyclonal Antibody
Other nameAnti-Btk (Ser180) Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetBtk (Ser180)
Short nameAnti-Btk (Ser180) (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-Bruton agammaglobulinemia tyrosine kinase (Ser180) (polyclonal)
Alternative techniquepolyclonals

Similar products

Subscribe to our Newsletter