| Category | Antibody |
| Concentration | pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form | Antigen affinity purified |
| Conjugation | Unconjugated |
| Clone | Polyclonal antibody |
| Recognised antigen | Anti-CD34 |
| Host animal | Rabbit (Oryctolagus cuniculus) |
| Clonality | Polyclonal (rabbit origin) |
| Species reactivity | Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications | ELISA, WB, FACS |
| Recommended dilutions | Flow Cytometry: 1:25, Western blot: 1:1000 |
| Notes | Titration of the anti-CD34 antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use | This anti-CD34 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot # | P28906 |
| Purity | Antigen affinity |
| Description | Could act as a scaffold for the attachment of lineage specific glycans, Presents carbohydrate ligands to selectins, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components, CD34 is a possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells |
| Immunogen | This anti-CD34 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 356-390 amino acids from the C-terminal region of human CD34 |
| Storage | Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the anti-CD34 antibody and store frozen at -20 deg |
| Properties | Stem cell or hematopoietic marker, The most used affinity clone is QBEND10, We compare the affinity to this clone, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target | CD34 |
| Short name | Anti-CD34 Antibody |
| Technique | antibodies against human proteins, antibodies for, Antibody |
| Alternative name | antibody to-CD34 molecule (Antibody to) |
| Alternative technique | antibodies |
| Alternative to gene target | CD34 and IDBG-106399 and ENSG00000174059 and 947, CD34 and IDBG-638449 and ENSBTAG00000010562 and 281051, Cd34 and IDBG-209317 and ENSMUSG00000016494 and 12490, Extracellular, sulfate binding, this GO :0001894 and tissue homeostasis and biological process this GO :0001935 and endothelial cell proliferation and biological process this GO :0003094 and glomerular filtration and biological process this GO :0003158 and endothelium development and biological process this GO :0005576 and extracellular region and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005764 and lysosome and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006536 and glutamate metabolic process and biological process this GO :0007155 and cell adhesion and biological process this GO :0007165 and signal transduction and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0008217 and regulation of blood pressure and biological process this GO :0008283 and cell proliferation and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0009925 and basal plasma membrane and cellular component this GO :0009986 and cell surface and cellular component this GO :0010628 and positive regulation of gene expression and biological process this GO :0010629 and negative regulation of gene expression and biological process this GO :0016020 and membrane and cellular component this GO :0016324 and apical plasma membrane and cellular component this GO :0016337 and single organismal cell-cell adhesion and biological process this GO :0030097 and hemopoiesis and biological process this GO :0030195 and negative regulation of blood coagulation and biological process this GO :0030246 and carbohydrate binding and molecular function this GO :0032720 and negative regulation of tumor necrosis factor production and biological process this GO :0032733 and positive regulation of interleukin-10 production and biological process this GO :0035759 and mesangial cell-matrix adhesion and biological process this GO :0036053 and glomerular endothelium fenestra and cellular component this GO :0038001 and paracrine signaling and biological process this GO :0042482 and positive regulation of odontogenesis and biological process this GO :0043199 and sulfate binding and molecular function this GO :0045019 and negative regulation of nitric oxide biosynthetic process and biological process this GO :0045171 and intercellular bridge and cellular component this GO :0045766 and positive regulation of angiogenesis and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0048870 and cell motility and biological process this GO :0050776 and regulation of immune response and biological process this GO :0050900 and leukocyte migration and biological process this GO :0060290 and transdifferentiation and biological process this GO :0061042 and vascular wound healing and biological process this GO :0071425 and hematopoietic stem cell proliferation and biological process this GO :0071636 and positive regulation of transforming growth factor beta production and biological process this GO :0071657 and positive regulation of granulocyte colony-stimulating factor production and biological process this GO :0071944 and cell periphery and cellular component this GO :0071971 and extracellular vesicular exosome assembly and biological process this GO :0072011 and glomerular endothelium development and biological process this GO :0072089 and stem cell proliferation and biological process this GO :0072254 and metanephric glomerular mesangial cell differentiation and biological process this GO :1900035 and negative regulation of cellular response to heat and biological process this GO :1900038 and negative regulation of cellular response to hypoxia and biological process this GO :1900041 and negative regulation of interleukin-2 secretion and biological process this GO :1900168 and positive regulation of glial cell line-derived neurotrophic factor secretion and biological process this GO :1901215 and negative regulation of neuron death and biological process this GO :2001214 and positive regulation of vasculogenesis and biological process, this GO :0008134: transcription factor binding, this GO :0008134: transcription factor binding and also this GO :0030246: carbohydrate binding and also this GO :0043199: sulfate binding, this GO :0030246: carbohydrate binding, this GO :0043199: sulfate binding, CD34 molecule |
| Identity | 1662 |
| Gene | CD34 |
| Long gene name | CD34 molecule |
| Synonyms gene name | CD34 antigen |
| Locus | 1q32, 2 |
| Discovery year | 1990-09-10 |
| GenBank acession | M81104 |
| Entrez gene record | 947 |
| Pubmed identfication | 1370171 1374051 |
| RefSeq identity | NM_001773 |
| Classification | CD molecules |
| Havana BLAST/BLAT | OTTHUMG00000036565 |