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Anti-Cdc25B (Ser186) (Polyclonal), ALEXA Fluor 594

Anti-Cdc25B (Ser186) (Polyclonal), ALEXA Fluor 594 size: 100 microliters 516

Price 516
Size 100 microliters
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideCdc25B Ser186
SpecificityThis antibody reacts specifically with Cdc25B (Ser186)
ModificationPhosphorylation
Modification siteSer186
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsCytoplasm
Antigen SourceKLH conjugated synthetic phosphopeptide derived from human Cdc25B around the phosphorylation site of Ser186
Gene ID994
Swiss ProtP30305
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information Directly dephosphorylates CDK1 and stimulates its kinase activity, Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner, The three isoforms seem to have a different level of activity, Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Synonyms CDC25B, CDC25HU2, Dual specificity phosphatase Cdc25B, M-phase inducer phosphatase 2
Also known asCdc25B (Ser186) Polyclonal Antibody
Other nameAnti-Cdc25B (Ser186) Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetCdc25B (Ser186)
Short nameAnti-Cdc25B (Ser186) (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-Cdc25B (Ser186) (polyclonal)
Alternative techniquepolyclonals

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