Name : Anti-CDC27 (Thr244) (Polyclonal), ALEXA Fluor 594
Supplier : BIOSS POLYCLONAL ANTIBODIES
Price :516
SKU : GEN8845936441
| Type | Conjugated Primary Antibody |
| Conjugated with | 594, ALEXA FLUOR® |
| Host organism | Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide | CDC27 Thr244 |
| Specificity | This antibody reacts specifically with CDC27 (Thr244) |
| Modification | Phosphorylation |
| Modification site | Thr244 |
| Clonality | Polyclonal Antibody |
| Clone | Polyclonal Antibodies |
| Concentration | 1ug per 1ul |
| Subcellular locations | N/A |
| Antigen Source | KLH conjugated synthetic phosphopeptide derived from human CDC27 around the phosphorylation site of Thr244 |
| Gene ID | 996 |
| Swiss Prot | N/A |
| Applications | IF(IHC-P) |
| Applications with corresponding dilutions | IF(IHC-P)(1:50-200) |
| Cross reactive species | Human (Homo sapiens) |
| Cross Reactive Species details | However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information | APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins, Alternatively spliced transcript variants encoding different isoforms have been found for this gene, Jul 2008], This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), This protein is a component of anaphase-promoting complex (APC), This protein was shown to interact with mitotic checkpoint proteins including Mad2, [provided by RefSeq, a protein domain important for protein-protein interaction, and the gene product of Schizosaccharomyces pombe nuc 2, and thus may be involved in controlling the timing of mitosis, p55CDC and BUBR1, which is composed of eight protein subunits and highly conserved in eucaryotic cells, The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27 |
| Purification method | Purified by Protein A |
| Storage | 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission | 590nm/617nm |
| Synonyms | APC3 ANAPC3 NUC2 |
| Also known as | CDC27 (Thr244) Polyclonal Antibody |
| Other name | Anti-CDC27 (Thr244) Polyclonal |
| Advisory | For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties | For facs or microscopy Alexa 1 conjugate |
| Conjugation | Alexa Fluor |
| Group | Polyclonals and antibodies |
| About | The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target | CDC27 (Thr244) |
| Short name | Anti-CDC27 (Thr244) (Polyclonal) Fluor 594 |
| Technique | Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different  |
| Label | ALEXA |
| Alternative name | ALEXA Fluor 594, antibody to-CDC27 (Thr244) (polyclonal) |
| Alternative technique | polyclonals |
| Identity | 1728 |
| Gene | CDC27 |
| Long gene name | cell division cycle 27 |
| Synonyms gene | D0S1430E D17S978E |
| Synonyms gene name | cell division cycle 27 cell division cycle 27 homolog (S, cerevisiae) |
| Synonyms name | anaphase promoting complex subunit 3 |
| Locus | 17q21, 32 |
| Discovery year | 1994-02-23 |
| GenBank acession | U00001 |
| Entrez gene record | 996 |
| Pubmed identfication | 8234252 |
| Classification | Anaphase promoting complex Tetratricopeptide repeat domain containing |
| Havana BLAST/BLAT | OTTHUMG00000166429 |