Filters

Anti-CHEK1(Ser317) (Polyclonal), ALEXA Fluor 594

Anti-CHEK1(Ser317) (Polyclonal), ALEXA Fluor 594 size: 100 microliters 516

Supplier BIOSS POLYCLONAL ANTIBODIES
Price 516
Size 100 microliters
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideCHEK1 Ser317
SpecificityCHEK1(Ser317), This phosphorylation site is homologous to that of Ser317 in Mouse and Rat
ModificationPhosphorylation
Modification siteSer317
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locations Nucleus, Cytoplasm
Antigen SourceKLH conjugated synthetic phosphopeptide derived from human CHEK1 around the phosphorylation site of Ser317
Gene ID1111
Swiss ProtO14757
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A, 'Ser-82' and 'Ser-88' by NEK11, Also phosphorylates NEK6, Also promotes repair of DNA cross-links through phosphorylation of FANCE, Binds to and phosphorylates CDC25A, Binds to and phosphorylates RAD51 at 'Thr-309', Binds to and phosphorylates TLK1 at 'Ser-743', CDC25B and CDC25C, Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression, May also negatively regulate cell cycle progression during unperturbed cell cycles, May also play a role in replication fork maintenance through regulation of PCNA, Phosphorylates multiple sites within the C-terminus of TP53, Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C, Phosphorylation of CDC25A at 'Ser-76', Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', Recognizes the substrate consensus sequence [R-X-X-S/T], This may enhance chromatin assembly both in the presence or absence of DNA damage, This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome, thereby promoting DNA repair by homologous recombination, which is required for polyubiquitination and degradation of CDCD25A, which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular proliferation, which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin, Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Synonyms CHK1
Also known asCHEK1(Ser317) Polyclonal Antibody
Other nameAnti-CHEK1(Ser317) Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetCHEK1(Ser317)
Short nameAnti-CHEK1(Ser317) (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-CHEK1(Ser317) (polyclonal)
Alternative techniquepolyclonals
Identity 1925
Gene CHEK1
Long gene name checkpoint kinase 1
Synonyms gene name CHK1 (checkpoint, S, pombe) , pombe) homolog CHK1 checkpoint homolog (S
Locus 11q24, 2
Discovery year 1998-04-21
GenBank acession AF016582 BC017575
Entrez gene record 1111
Pubmed identfication 9278511 9382850
RefSeq identity NM_001274
Havana BLAST/BLAT OTTHUMG00000165853

Similar products

Subscribe to our Newsletter