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Anti-Collagen III (Polyclonal), ALEXA Fluor 594

Anti-Collagen III (Polyclonal), ALEXA Fluor 594 size: 100 microliters 489

Price 489
Size 100 microliters
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideCollagen III
Specificity it will only recognize the full length Collagen III protein and not the mature form, Collagen III, This antibody is produced with an immunogen contained within the COLFI propeptide region
ModificationNo modification has been applied to this antibody
Modification siteNone
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsSecreted
Antigen SourceKLH conjugated synthetic peptide derived from human Collagen alpha 1(III) chain (C-terminal propeptide)
Gene ID1281
Swiss ProtP02461
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information Involved in regulation of cortical development, Is the major ligand of GPR56 in the developing brain and binding to GPR56 inhibits neuronal migration and activates the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12, Collagen type III occurs in most soft connective tissues along with type I collagen
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Synonyms C-P4Halpha(III)
Also known asCollagen III Polyclonal Antibody
Other nameAnti-Collagen III Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetCollagen III
Short nameAnti-Collagen III (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-Collagen III (polyclonal)
Alternative techniquepolyclonals
Identity 30135
Gene P4HA3
Long gene name prolyl 4-hydroxylase subunit alpha 3
Synonyms gene name 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III , alpha polypeptide III prolyl 4-hydroxylase, procollagen-proline
Synonyms name collagen prolyl 4-hydroxylase alpha(III)
Locus 11q13, 4
Discovery year 2004-05-12
GenBank acession AY327887
Entrez gene record 283208
Pubmed identfication 14500733
RefSeq identity NM_182904
Havana BLAST/BLAT OTTHUMG00000165254

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