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Anti-Collagen IV (Polyclonal), ALEXA Fluor 594

Anti-Collagen IV (Polyclonal), ALEXA Fluor 594 size: 100 microliters 489

Price 489
Size 100 microliters
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideCollagen IV
SpecificityCollagen IV, This antibody has 79% cross reactivity with Collagen alpha-5(IV) chain
ModificationNo modification has been applied to this antibody
Modification siteNone
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsSecreted
Antigen SourceKLH conjugated synthetic peptide derived from mouse Collagen alpha-1(IV) chain
Gene ID12826
Swiss ProtP02463
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information Arresten, Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation, Ligand for alpha1/beta1 integrin (By similarity), Specifically inhibits endothelial cell proliferation, The C-terminal half is found to possess the anti-angiogenic activity, comprising the C-terminal NC1 domain, forming a 'chicken-wire' meshwork together with laminins, inhibits angiogenesis and tumor formation, migration and tube formation, proteoglycans and entactin/nidogen, Type IV collagen is the major structural component of glomerular basement membranes (GBM)
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Synonyms FLJ22259 DKFZp686I14213
Also known asCollagen IV Polyclonal Antibody
Other nameAnti-Collagen IV Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetCollagen IV
Short nameAnti-Collagen IV (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-Collagen IV (polyclonal)
Alternative techniquepolyclonals
Identity 2203
Gene COL4A2
Long gene name collagen type IV alpha 2 chain
Synonyms gene name collagen type IV alpha 2
Synonyms name canstatin collagen type IV alpha 2
Locus 13q34
Discovery year 2001-06-22
GenBank acession AK025912
Entrez gene record 1284
Pubmed identfication 2439508 3025878
RefSeq identity NM_001846
Classification Collagens
Havana BLAST/BLAT OTTHUMG00000017344

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