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Anti-DNCLI1 (Polyclonal), Cy3

Anti-DNCLI1 (Polyclonal), Cy3 size: 100 microliters 516

Price 516
Size 100 microliters
TypeConjugated Primary Antibody
Conjugated withCy3
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideDNCLI1
SpecificityThis antibody reacts specifically with DNCLI1 Cy3
ModificationNo modification has been applied to this antibody
Modification siteNone
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsCytoplasm
Antigen SourceKLH conjugated synthetic peptide derived from human DNCLI1
Gene ID51143
Swiss ProtQ9Y6G9
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules, Is required for progress throuh the spindle assembly checkpoint, May play a role in binding dynein to membranous organelles or chromosomes, Probably involved in the microtubule-dependent transport of pericentrin, The phosphorylated form appears to be involved in the selective removal of MAD1L1 and MAD1L2 but not BUB1B from kinetochores, Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission512, 550nm/570, 615nm
Also known asDNCLI1 Polyclonal Antibody Cy3
Other nameAnti-DNCLI1 Polyclonal Cy3
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
Properties 1 of the Anti-DNCLI1 (Polyclonal) can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC, This Bioss Polyclonal Antibodies antibody is well suited for fluorescent microscopy, Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser
Conjugationcy3 conjugation kit
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetDNCLI1
Short nameAnti-DNCLI1 (Polyclonal)
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelCy3
Alternative name cyanine 3, antibody to-DNCLI1 (polyclonal)
Alternative techniquepolyclonals
Identity 18745
Gene DYNC1LI1
Long gene name dynein cytoplasmic 1 light intermediate chain 1
Synonyms gene DNCLI1
Synonyms gene name cytoplasmic, dynein, light intermediate polypeptide 1
Locus 3p22, 3
Discovery year 2002-06-12
GenBank acession AF078849
Entrez gene record 51143
Pubmed identfication 16260502
RefSeq identity NM_016141
Classification Dyneins, cytoplasmic
Havana BLAST/BLAT OTTHUMG00000130750

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