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Anti-DNCLI1 (Polyclonal), FITC

Anti-DNCLI1 (Polyclonal), FITC size: 100 microliters 489

Supplier BIOSS POLYCLONAL ANTIBODIES
Price 489
Size 100 microliters
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
TypeConjugated Primary Antibody
Conjugated withFITC
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideDNCLI1
SpecificityThis antibody reacts specifically with DNCLI1 FITC
ModificationNo modification has been applied to this antibody
Modification siteNone
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsCytoplasm
Antigen SourceKLH conjugated synthetic peptide derived from human DNCLI1
Gene ID51143
Swiss ProtQ9Y6G9
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules, Is required for progress throuh the spindle assembly checkpoint, May play a role in binding dynein to membranous organelles or chromosomes, Probably involved in the microtubule-dependent transport of pericentrin, The phosphorylated form appears to be involved in the selective removal of MAD1L1 and MAD1L2 but not BUB1B from kinetochores, Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission494nm/518nm
Also known asDNCLI1 Polyclonal Antibody FITC
Other nameAnti-DNCLI1 Polyclonal FITC
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
Properties Bioss Polyclonal Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, This Bioss Polyclonal Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS
ConjugationAnti-FITC Antibody
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetDNCLI1
Short nameAnti-DNCLI1 (Polyclonal) FITC
Technique Fluorescein, Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelFITC
Alternative name fluorecein, antibody to-DNCLI1 (polyclonal)
Alternative techniquepolyclonals
Identity 18745
Gene DYNC1LI1
Long gene name dynein cytoplasmic 1 light intermediate chain 1
Synonyms gene DNCLI1
Synonyms gene name cytoplasmic, dynein, light intermediate polypeptide 1
Locus 3p22, 3
Discovery year 2002-06-12
GenBank acession AF078849
Entrez gene record 51143
Pubmed identfication 16260502
RefSeq identity NM_016141
Classification Dyneins, cytoplasmic
Havana BLAST/BLAT OTTHUMG00000130750

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