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Anti-E2F3 (Polyclonal), ALEXA Fluor 594

Anti-E2F3 (Polyclonal), ALEXA Fluor 594 size: 100 microliters 489

Price 489
Size 100 microliters
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideE2F3
SpecificityThis antibody reacts specifically with E2F3
ModificationNo modification has been applied to this antibody
Modification siteNone
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsNucleus
Antigen SourceKLH conjugated synthetic peptide derived from human E2F3
Gene ID1871
Swiss ProtO00716
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication, E2F3 binds specifically to RB1 in a cell-cycle dependent manner, Inhibits adipogenesis, The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase, probably through the repression of CEBPA binding to its target gene promoters (By similarity), Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Also known asE2F3 Polyclonal Antibody
Other nameAnti-E2F3 Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetE2F3
Short nameAnti-E2F3 (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-E2F3 (polyclonal)
Alternative techniquepolyclonals
Identity 3115
Gene E2F3
Long gene name E2F transcription factor 3
Locus 6p22, 3
Discovery year 1994-08-31
GenBank acession Y10479
Entrez gene record 1871
Pubmed identfication 8246996
Classification E2F transcription factors
Havana BLAST/BLAT OTTHUMG00000016389

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