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Anti-eIF4E (Polyclonal), ALEXA Fluor 594

Anti-eIF4E (Polyclonal), ALEXA Fluor 594 size: 100 microliters 489

Supplier BIOSS POLYCLONAL ANTIBODIES
Price 489
Size 100 microliters
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/Peptidee4E
SpecificityThis antibody reacts specifically with eIF4E
ModificationNo modification has been applied to this antibody
Modification siteNone
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsN/A
Antigen SourceKLH conjugated synthetic peptide derived from human eIF4E
Gene ID1977
Swiss ProtN/A
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information Phosphorylation also enhances the interaction of eIF4E with eIF4G, Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA, Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription, eIF4E also influences the overall rate of translation, eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs, eIF4E phosphorylation is correlated with increased translational rate in a number of cell types, which form a complex known as eIF4F, eIF4E
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Synonyms EIF4E1
Also known aseIF4E Polyclonal Antibody
Other nameAnti-eIF4E Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targeteIF4E
Short nameAnti-eIF4E (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-eIF4E (polyclonal)
Alternative techniquepolyclonals
Identity 3287
Gene EIF4E
Long gene name eukaryotic translation initiation factor 4E
Synonyms gene EIF4EL1 EIF4F
Locus 4q23
Discovery year 1991-07-09
GenBank acession M15353
Entrez gene record 1977
Pubmed identfication 9330633 1916814
RefSeq identity NM_001968
Havana BLAST/BLAT OTTHUMG00000161090

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