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Anti-eIF4E (Ser209) (Polyclonal), ALEXA Fluor 594

Anti-eIF4E (Ser209) (Polyclonal), ALEXA Fluor 594 size: 100 microliters 516

Price 516
Size 100 microliters
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideeIF4E Ser209
SpecificityThis antibody reacts specifically with eIF4E (Ser209)
ModificationPhosphorylation
Modification siteSer209
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsHelical
Antigen SourceKLH conjugated synthetic phosphopeptide derived from human eIF4E around the phosphorylation site of Ser209 [SG(p-S)TT]
Gene ID1977
Swiss ProtP06730
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression, In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap, Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Synonyms AUTS19, EIF4E1, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4E, eIF-4F 25 kDa subunit, eIF4E, mRNA cap-binding protein, CBP
Also known aseIF4E (Ser209) Polyclonal Antibody
Other nameAnti-eIF4E (Ser209) Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targeteIF4E (Ser209)
Short nameAnti-eIF4E (Ser209) (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-eIF4E (Ser209) (polyclonal)
Alternative techniquepolyclonals

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