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Anti-EN2/Engrailed 2 (Polyclonal), ALEXA Fluor 594

Anti-EN2/Engrailed 2 (Polyclonal), ALEXA Fluor 594 size: 100 microliters 489

Price 489
Size 100 microliters
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideEN2/Engrailed 2
SpecificityThis antibody reacts specifically with EN2/Engrailed 2
ModificationNo modification has been applied to this antibody
Modification siteNone
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsCytoplasm
Antigen SourceKLH conjugated synthetic peptide derived from human EN2/Engrailed 2
Gene IDN/A
Swiss ProtN/A
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information Different mutations in the mouse homologs, During early brain development mouse EN-2 is expressed in a broad band across most of the mid-hindbrain region, EN-1 and EN-2, EN-1 and EN-2 regulate expression of a-synuclein, EN-1 is highly expressed by essentially all dopaminergic neurons in the substantia nigra and ventral tegmentum, EN-2 is also expressed in mouse myoblasts and has been assiciated with cerebellar hypoplasia, EN2, Human EN-1 and EN-2 are homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system, In Drosophila, a gene that is genetically linked to Parkinsonâ, a murine homolog of the Drosophila homeobox gene engrailed (EN), is required for midbrain and cerebellum development and dorsal/ventral patterning of the limbs as well as apical ectodermal ridge formation, produce different developmental defects that frequently are lethal, the EN gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments, &euro, &trade, The engrailed-2 gene, s disease
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Also known asEN2/Engrailed 2 Polyclonal Antibody
Other nameAnti-EN2/Engrailed 2 Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetEN2/Engrailed 2
Short nameAnti-EN2/Engrailed 2 (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-EN2/Engrailed 2 (polyclonal)
Alternative techniquepolyclonals
Identity 3343
Gene EN2
Long gene name engrailed homeobox 2
Locus 7q36, 3
Discovery year 1988-08-31
Entrez gene record 2020
RefSeq identity NM_001427
Classification NKL subclass homeoboxes and pseudogenes
Havana BLAST/BLAT OTTHUMG00000151354

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