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Anti-EPHA2 (Polyclonal), Cy5.5
Anti-EPHA2 (Polyclonal), Cy5.5 size: 100 microliters 516
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BIOSS POLYCLONAL ANTIBODIES
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516
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100 microliters
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| Type | Conjugated Primary Antibody |
| Conjugated with | 5, Cy5 |
| Host organism | Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide | EPHA2 |
| Specificity | 5, This antibody reacts specifically with EPHA2 Cy5 |
| Modification | No modification has been applied to this antibody |
| Modification site | None |
| Clonality | Polyclonal Antibody |
| Clone | Polyclonal Antibodies |
| Concentration | 1ug per 1ul |
| Subcellular locations | Extracellular |
| Antigen Source | KLH conjugated synthetic peptide derived from human EPHA2 |
| Gene ID | 1969 |
| Swiss Prot | P29317 |
| Applications | IF(IHC-P) |
| Applications with corresponding dilutions | IF(IHC-P)(1:50-200) |
| Cross reactive species | Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details | However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information | Activated by the ligand ephrin-A1/EFNA1 regulates migration, During development, Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance, Involved for instance in angiogenesis, May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration, Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling, With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development, integrin-mediated adhesion, leading to contact-dependent bidirectional signaling into neighboring cells, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues, proliferation and differentiation of cells, respectively) signaling pathway, Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells |
| Purification method | Purified by Protein A |
| Storage | 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission | 675nm/694nm |
| Also known as | 5, EPHA2 Polyclonal Antibody Cy5 |
| Other name | 5, Anti-EPHA2 Polyclonal Cy5 |
| Advisory | For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties | Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor |
| Conjugation | Cy5, 5, 5, Cy5 |
| Conjugated | These antibodies are excite for emission at 678nm and detected at a 694 , nm wavelengths |
| Group | Polyclonals and antibodies |
| About | The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target | EPHA2 |
| Short name | Anti-EPHA2 (Polyclonal) |
| Technique | Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label | 5, Cy5 |
| Alternative name | cyanine 5, 5, antibody to-EPH receptor A2 (polyclonal) |
| Alternative technique | polyclonals |
| Alternative to gene target | ARCC2 and CTPA and CTPP1 and CTRCT6 and ECK, Cell surfaces, EPHA2 and IDBG-645870 and ENSBTAG00000000815 and 512798, EPHA2 and IDBG-91407 and ENSG00000142627 and 1969, Epha2 and IDBG-202005 and ENSMUSG00000006445 and 13836, this GO :0001501 and skeletal system development and biological process this GO :0001525 and angiogenesis and biological process this GO :0001568 and blood vessel development and biological process this GO :0001570 and vasculogenesis and biological process this GO :0001649 and osteoblast differentiation and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0004714 and transmembrane receptor protein tyrosine kinase activity and molecular function this GO :0005003 and ephrin receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0005925 and focal adhesion and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0007155 and cell adhesion and biological process this GO :0007169 and transmembrane receptor protein tyrosine kinase signaling pathway and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0008630 and intrinsic apoptotic signaling pathway in response to DNA damage and biological process this GO :0010591 and regulation of lamellipodium assembly and biological process this GO :0014028 and notochord formation and biological process this GO :0016032 and viral process and biological process this GO :0016477 and cell migration and biological process this GO :0016772 and transferase activity, this GO :0004672: protein kinase activity, this GO :0004672: protein kinase activity and also this GO :0004713: protein tyrosine kinase activity and also this GO :0004714: transmembrane receptor protein tyrosine kinase activity and also this GO :0005003: ephrin receptor activity and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0016772: transferase activity, this GO :0004713: protein tyrosine kinase activity, this GO :0004714: transmembrane receptor protein tyrosine kinase activity, this GO :0005003: ephrin receptor activity, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0016772: transferase activity, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups, transferring phosphorus-containing groups and molecular function this GO :0018108 and peptidyl-tyrosine phosphorylation and biological process this GO :0021915 and neural tube development and biological process this GO :0030182 and neuron differentiation and biological process this GO :0030216 and keratinocyte differentiation and biological process this GO :0030316 and osteoclast differentiation and biological process this GO :0031256 and leading edge membrane and cellular component this GO :0031258 and lamellipodium membrane and cellular component this GO :0032587 and ruffle membrane and cellular component this GO :0032863 and activation of Rac GTPase activity and biological process this GO :0033598 and mammary gland epithelial cell proliferation and biological process this GO :0033628 and regulation of cell adhesion mediated by integrin and biological process this GO :0036342 and post-anal tail morphogenesis and biological process this GO :0043491 and protein kinase B signaling and biological process this GO :0043535 and regulation of blood vessel endothelial cell migration and biological process this GO :0045765 and regulation of angiogenesis and biological process this GO :0046849 and bone remodeling and biological process this GO :0048013 and ephrin receptor signaling pathway and biological process this GO :0048320 and axial mesoderm formation and biological process this GO :0048570 and notochord morphogenesis and biological process this GO :0051898 and negative regulation of protein kinase B signaling and biological process this GO :0060035 and notochord cell development and biological process this GO :0060326 and cell chemotaxis and biological process this GO :0060444 and branching involved in mammary gland duct morphogenesis and biological process this GO :0070309 and lens fiber cell morphogenesis and biological process this GO :0070372 and regulation of ERK1 and ERK2 cascade and biological process this GO :0070848 and response to growth factor and biological process this GO :0090004 and positive regulation of establishment of protein localization to plasma membrane and biological process, EPH receptor A2 |
| Identity | 3386 |
| Gene | EPHA2 |
| Long gene name | EPH receptor A2 |
| Synonyms gene | ECK |
| Synonyms gene name | EphA2 |
| Locus | 1p36, 13 |
| Discovery year | 1991-08-07 |
| GenBank acession | BC037166 |
| Entrez gene record | 1969 |
| Pubmed identfication | 9119409 |
| RefSeq identity | NM_004431 |
| Classification | Sterile alpha motif domain containing Fibronectin type III domain containing EPH receptors |
| Havana BLAST/BLAT | OTTHUMG00000009527 |