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Anti-ERK1(Thr197 +Thr202) (Polyclonal), ALEXA Fluor 594

Anti-ERK1(Thr197 +Thr202) (Polyclonal), ALEXA Fluor 594 size: 100 microliters 516

Price 516
Size 100 microliters
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideERK1 Thr197 +Thr202
SpecificityThis antibody reacts specifically with ERK1(Thr197 +Thr202)
ModificationPhosphorylation
Modification siteThr197 +Thr202
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsN/A
Antigen SourceKLH conjugated synthetic phosphopeptide derived from human ERK1 around the phosphorylation site of Thr197/Thr202
Gene ID5595
Swiss ProtN/A
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information MAP kinases, The activation of this kinase requires its phosphorylation by upstream kinases, Two alternatively spliced transcript variants encoding the same protein, Upon activation, act as an integration point for multiple biochemical signals, also known as extracellular signal-regulated kinases (ERKs), and are involved in a wide variety of cellular processes such as proliferation, but differing in the UTRs, differentiation, have been reported for this gene, this kinase translocates to the nucleus of the stimulated cells, transcription regulation and development, where it phosphorylates nuclear targets, The protein encoded by this gene is a member of the MAP kinase family
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Synonyms ERK1 p44mapk p44erk1
Also known asERK1(Thr197 +Thr202) Polyclonal Antibody
Other nameAnti-ERK1(Thr197 +Thr202) Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetERK1(Thr197 +Thr202)
Short nameAnti-ERK1(Thr197 +Thr202) (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-ERK1(Thr197 +Thr202) (polyclonal)
Alternative techniquepolyclonals
Identity 6877
Gene MAPK3
Long gene name mitogen-activated protein kinase 3
Synonyms gene PRKM3
Locus 16p11, 2
Discovery year 1993-11-05
GenBank acession M84490
Entrez gene record 5595
Pubmed identfication 9628824
Classification Mitogen-activated protein kinases
Havana BLAST/BLAT OTTHUMG00000132149

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