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Anti-EYA1 (Polyclonal), Cy3
Anti-EYA1 (Polyclonal), Cy3 size: 100 microliters 516
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BIOSS POLYCLONAL ANTIBODIES
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516
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100 microliters
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| Type | Conjugated Primary Antibody |
| Conjugated with | Cy3 |
| Host organism | Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide | EYA1 |
| Specificity | This antibody reacts specifically with EYA1 Cy3 |
| Modification | No modification has been applied to this antibody |
| Modification site | None |
| Clonality | Polyclonal Antibody |
| Clone | Polyclonal Antibodies |
| Concentration | 1ug per 1ul |
| Subcellular locations | Nucleus, Cytoplasm |
| Antigen Source | KLH conjugated synthetic peptide derived from human EYA1 |
| Gene ID | 2138 |
| Swiss Prot | Q99502 |
| Applications | IF(IHC-P) |
| Applications with corresponding dilutions | IF(IHC-P)(1:50-200) |
| Cross reactive species | Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details | However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information | 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress, Has also phosphatase activity with proteins phosphorylated on Ser and Thr residues (in vitro), Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis, Required for normal embryonic development of the craniofacial and trunk skeleton, SIX4 and SIX5, Together with SIX1, Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1, and probably also for SIX2, in this it is functionally redundant with EYA2, it plays an important role in hypaxial muscle development, kidneys and ears, Functions both as protein phosphatase and as transcriptional coactivator for SIX1 |
| Purification method | Purified by Protein A |
| Storage | 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission | 512, 550nm/570, 615nm |
| Also known as | EYA1 Polyclonal Antibody Cy3 |
| Other name | Anti-EYA1 Polyclonal Cy3 |
| Advisory | For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties | 1 of the Anti-EYA1 (Polyclonal) can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC, This Bioss Polyclonal Antibodies antibody is well suited for fluorescent microscopy, Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser |
| Conjugation | cy3 conjugation kit |
| Group | Polyclonals and antibodies |
| About | The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target | EYA1 |
| Short name | Anti-EYA1 (Polyclonal) |
| Technique | Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label | Cy3 |
| Alternative name | cyanine 3, antibody to-eyes absent homolog 1 (Drosophila) (polyclonal) |
| Alternative technique | polyclonals |
| Alternative to gene target | BOP and BOR and BOS1 and OFC1, DNA-templated and biological process this GO :0006470 and protein dephosphorylation and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0007389 and pattern specification process and biological process this GO :0007605 and sensory perception of sound and biological process this GO :0009653 and anatomical structure morphogenesis and biological process this GO :0009887 and organ morphogenesis and biological process this GO :0010212 and response to ionizing radiation and biological process this GO :0014706 and striated muscle tissue development and biological process this GO :0016576 and histone dephosphorylation and biological process this GO :0016925 and protein sumoylation and biological process this GO :0034613 and cellular protein localization and biological process this GO :0035088 and establishment or maintenance of apical/basal cell polarity and biological process this GO :0035335 and peptidyl-tyrosine dephosphorylation and biological process this GO :0035909 and aorta morphogenesis and biological process this GO :0042471 and ear morphogenesis and biological process this GO :0042472 and inner ear morphogenesis and biological process this GO :0042473 and outer ear morphogenesis and biological process this GO :0042474 and middle ear morphogenesis and biological process this GO :0045165 and cell fate commitment and biological process this GO :0045664 and regulation of neuron differentiation and biological process this GO :0045739 and positive regulation of DNA repair and biological process this GO :0045747 and positive regulation of Notch signaling pathway and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046872 and metal ion binding and molecular function this GO :0048665 and neuron fate specification and biological process this GO :0048704 and embryonic skeletal system morphogenesis and biological process this GO :0048752 and semicircular canal morphogenesis and biological process this GO :0048856 and anatomical structure development and biological process this GO :0050679 and positive regulation of epithelial cell proliferation and biological process this GO :0060037 and pharyngeal system development and biological process this GO :0060487 and lung epithelial cell differentiation and biological process this GO :0071599 and otic vesicle development and biological process this GO :0071600 and otic vesicle morphogenesis and biological process this GO :0072513 and positive regulation of secondary heart field cardioblast proliferation and biological process this GO :0090103 and cochlea morphogenesis and biological process this GO :2001240 and negative regulation of extrinsic apoptotic signaling pathway in absence of ligand and biological process, EYA1 and IDBG-25014 and ENSG00000104313 and 2138, EYA1 and IDBG-644711 and ENSBTAG00000011298 and 511188, Eya1 and IDBG-134456 and ENSMUSG00000025932 and 14048, metal ion binding, nuclei, this GO :0000132 and establishment of mitotic spindle orientation and biological process this GO :0001656 and metanephros development and biological process this GO :0001657 and ureteric bud development and biological process this GO :0001658 and branching involved in ureteric bud morphogenesis and biological process this GO :0003151 and outflow tract morphogenesis and biological process this GO :0003723 and RNA binding and molecular function this GO :0004725 and protein tyrosine phosphatase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006302 and double-strand break repair and biological process this GO :0006351 and transcription, this GO :0003723: RNA binding, this GO :0003723: RNA binding and also this GO :0004725: protein tyrosine phosphatase activity and also this GO :0005515: protein binding and also this GO :0046872: metal ion binding, this GO :0004725: protein tyrosine phosphatase activity, this GO :0005515: protein binding, this GO :0046872: metal ion binding, eyes absent homolog 1 (Drosophila) |
| Identity | 3519 |
| Gene | EYA1 |
| Long gene name | EYA transcriptional coactivator and phosphatase 1 |
| Synonyms gene | BOR |
| Synonyms gene name | eyes absent (Drosophila) homolog 1 eyes absent homolog 1 (Drosophila) |
| Locus | 8q13, 3 |
| Discovery year | 1996-12-12 |
| GenBank acession | AJ000098 |
| Entrez gene record | 2138 |
| Pubmed identfication | 9020840 |
| RefSeq identity | NM_000503 NM_172060 |
| Classification | EYA transcriptional coactivator and phosphatases |
| Havana BLAST/BLAT | OTTHUMG00000149894 |