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anti-FasL Antibody [A11]

anti-FasL Antibody [A11] size: 0.1 mg 180

Supplier PROSCIENCE
Price 180
Size 0.1 mg
Gene names APT1LG1, CD178, CD95 ligand, CD95-L, CD95L, Cd95l, Fas-L, Fasl, Faslg, Tnfsf6, Tumor necrosis factor ligand superfamily member 6, gld, Apt1lg1
Antibodie's labelunlabelled serum
Purity#8805, 95% in SDS-PAGE, FasL (monoclonal A11) antibody was purified to &
Ab raised inRat
TypeMonoclonal
MonoclonalA11
ImmunoglobulinIgM
AntigenFasL (monoclonal A11) antibody was made against a synthetic peptide corresponding to amino acids 196-220 of mouse FasL
Immuno application FLOW, immuno-histo-chemistry
Antibody advice 0, 0, 70%), 95%, After rinsing in PBS, EtOH 100% (2x10 min), Sections were incubated for 1 hr at room temperature (RT) with 20 ug/mL of A11 antibody in PBS, a FITC-conjugated anti-rat IgM was added for 30 min, block endogeneous peroxidase (0, cool down to RT 20 min, develop in appropriate substrate solution (DAB or AEC), fixed in 4% paraformaldehyde in PBS and used immediately, incubate in anti-rat IgM HRP, incubate with A11 20 ug/mL antibody, pH 6, rehydrate through graded EtOH (100%, rinse in H20, slides washed again in PBS and visualized using a fluorescence microscope, unmask epitopes by heating slides in citrate buffer (0, wash 1x in PBS, wash in PBS, wash in PBS, #956, 0) 2x5 min in microwave oven (650-750W), 01M, 05% Tween 20, 2% BSA, 6% H2O2 in Methanol 10 min), FasL antibody can be used for the detection of mouse FasL by flow cytometry and immunohistochemistry (frozen sections and paraffin sections), Method: Cryostat sections (6-8 &, Paraffin sections: Dewax in xylene (2x10 min), m) were air dried
Suspention, pH, azide02% sodium azide, PBS containing 0
Keep at stable for one year, FasL antibody can be stored at 4 grades C
Omim nr729462
Ncbi nrP41047
Vial with antibodyin solution
NCBI GENE nr14103
GENE symbolFasl
Antigene name member 6), Fas ligand (TNF superfamily
SourceMus musculus
Protein nrP41047
Description Freeze thaw will destroy a percentage in every cycle and should be avoided, C in a fridge short term in a concentrated dilution, This antibody needs to be stored at + 4°
PropertiesC, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by proscience they should be stored frozen at - 24°
Gene targetFasL [A11]
Short nameanti-FasL Antibody [A11]
Technique antibodies against human proteins, antibodies for, antibody to, Antibody
Alternative nameantibody to-FasL (Antibody to) [A11]
Alternative techniqueantibodies

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