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Anti-GAL4 (Polyclonal), ALEXA Fluor 594

Anti-GAL4 (Polyclonal), ALEXA Fluor 594 size: 100 microliters 489

Price 489
Size 100 microliters
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideGAL4
SpecificityThis antibody reacts specifically with GAL4
ModificationNo modification has been applied to this antibody
Modification siteNone
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsN/A
Antigen SourceKLH conjugated synthetic peptide derived from Saccharomyces cerevisiae GAL4
Gene IDN/A
Swiss ProtN/A
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive speciesYeast
Cross Reactive Species detailsSaccharomyces cerevisiae
Background information As the N-terminal 147 amino acid residues of GAL4 are sufficient to mediate specific and strong binding to DNA, Fields and coworkers have taken advantage of these findings by the development of a unique â, Fusions between GAL4 (amino acids 1-147) and activating domains from a variety of transcriptional regulatory proteins can activate transcription in yeast, This approach is facilitated by the finding that higher eukaryotes lack endogenous proteins that enhance transcription from the consensus GAL4-binding site, but are incapable of efficient transcriptional activation, insects and mammalian cells, plant, this protein fragment has frequently been used to confer specific DNA binding in experiments examining transcriptional activation functions of heterologous proteins, &euro, &euro, &oelig, The GAL4 protein of Saccharomyces cerevisiae is one of the most thoroughly characterized transcriptional activators, two-hybridâ,  system using GAL4 fusions in yeast to identify specific protein-protein interactions
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Synonyms GAL4
Also known asGAL4 Polyclonal Antibody
Other nameAnti-GAL4 Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetGAL4
Short nameAnti-GAL4 (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-GAL4 (polyclonal)
Alternative techniquepolyclonals
Identity 6565
Gene LGALS4
Long gene name galectin 4
Synonyms gene name 4 , galactoside-binding, lectin, soluble
Locus 19q13, 2
Discovery year 1993-08-24
Entrez gene record 3960
Pubmed identfication 8063692
RefSeq identity NM_006149
Classification Galectins
Havana BLAST/BLAT OTTHUMG00000182608

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