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anti-Gap-43 (phospho Ser41) Antibody

anti-Gap-43 (phospho Ser41) Antibody size: 0.1 mL 180

Supplier PROSCIENCE
Price 180
Size 0.1 mL
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
Gene info GAP-43 is thought to mediate at least some of these effects via interaction with actin, Importantly, There is also evidence from knockout animals that GAP-43 serves to amplify pathfinding signals from the growth cone, phosphorylation at Ser41 by Protein Kinase C modulates the interaction of GAP-43 with actin, GAP-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration
Gene names Axonal membrane protein GAP-43, Basp2
Antibodie's labelunlabelled serum
PurityAffinity Purified
Ab raised inRabbit
TypePolyclonal
Spicific toGap-43 antibody is specific for GAP-43 phosphorylated at Ser41
AntigenGAP-43 (Ser41) polyclonal antibody was made against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser41 of rat GAP-43
Immuno applicationWestern Blot
Antibody advice Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state, Applications include Dot blots (DB) and Western blots (WB), Human, Immunohistochemistry (IHC) application has not yet been determined, Immunolabeling of the GAP-43 protein in Western blots of rat brain is blocked by the Ser41 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide, Initial recommended range of dilutions: 1:500 to 1:2000, Note: the GAP-43 protein migrates on SDS-PAGE as a 50 kDa band, The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns, When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB, mouse and rat have 100% amino acid sequence identity with for the antigen used to raise the antibody, In some tissues the antibody also recognizes a higher molecular weight band that is also recognized by the Pan GAP-43 antibody and this band may be a GAP-43 aggregate or oligomer
Suspention, pH, azide 100 ug per mL BSA and 50% glycerol, 150 mM NaCl, 100 uL in 10 mM HEPES (pH 7, 5)
Keep at Stock solutions are stable for a minimum of 1 year at -20 grades C, but shorter term storage at -20 grades C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol, For long term storage -80 grades C is recommended
Works with Mouse, Rat, Human
Omim nr128102
Ncbi nrP07936
KDa50
Vial with antibodyin solution
NCBI GENE nr29423
GENE symbolGap43
Antigene namegrowth associated protein 43
SourceRattus norvegicus
Protein nrP07936
Description Freeze thaw will destroy a percentage in every cycle and should be avoided, C in a fridge short term in a concentrated dilution, This antibody needs to be stored at + 4°
PropertiesC, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by proscience they should be stored frozen at - 24°
Gene targetGap-43 (phospho Ser41)
Short nameanti-Gap-43 (phospho Ser41) Antibody
Technique antibodies against human proteins, antibodies for, antibody to, Antibody
Alternative nameantibody to-Gap-43 (phosphorilated Ser41) (Antibody to)
Alternative techniqueantibodies

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