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anti-Granzyme B Antibody [B18.1]

anti-Granzyme B Antibody [B18.1] size: 0.1 mg 180

Supplier PROSCIENCE
Price 180
Size 0.1 mg
Gene names C11, CCPI, CGL-1, CGL1, CSP-B, CSPB, CTLA1, CTSGL1, GRB, Granzyme B, HLP, SECT, CGL1
Antibodie's labelunlabelled serum
Purity#8805, 1) antibody was purified to &, 95% in SDS-PAGE, Granzyme B (monoclonal B18
Ab raised inMouse
TypeMonoclonal
Monoclonal1, B18
ImmunoglobulinIgG2a
Antigen amino acids 7-227, 1) antibody was made against recombinant human granzyme B, Granzyme B (monoclonal B18
Immuno application FLOW, immuno precipitation, immuno-cyto-chemistry, immuno-histo-chemistry, Western Blot
Antibody advice 0, 0, 0, 5% Fetal calf serum, After 3 washes in PBS, After rinsing in PBS, After washing in FACS buffer, Method: : Recombinant granzyme B was resolved by SDS-PAGE under reducing conditions, Method: Human T cells were plated onto polylysine treated glass slides, Method: Permeabilized human T cells (5 x 105) were incubated on ice for 30 min in 50 &, Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system, a FITC-conjugated anti-mouse IgG was added for 30 min, cells were analyzed by flow cytometry, fixed and permeabilized in methanol at -20oC for 5 min, immunocytochemistry, immunohistochemistry (frozen sections, immunoprecipitation and western blot, paraffin sections), slides washed again in PBS and visualized using a fluorescence microscope, slides were incubated for 1 hr at room temperature (RT) with 20 ug/mL of B18, then in acetone at -20oC for 30 sec, transferred to nitrocellulose and probed with the monoclonal anti-granzyme B antibody B18, #956, 02% azide) containing 1 ug of FITC- labeled B18, 1 antibody, 1 at 1 ug/mL, 1% BSA, 1% BSA, 4 antibody in PBS, Granzyme B antibody can be used for detection of human granzyme B by flow cytometry, l FACS buffer (PBS
Suspention, pH, azide02% sodium azide, PBS containing 0
Keep at stable for one year, Granzyme B antibody can be stored at 4 grades C
Omim nr317373361
Ncbi nrP10144
Vial with antibodyin solution
NCBI GENE nr3002
GENE symbolGZMB
Antigene name cytotoxic T-lymphocyte-associated serine esterase 1), granzyme B (granzyme 2
SourceHomo sapiens
Protein nrP10144
Description Freeze thaw will destroy a percentage in every cycle and should be avoided, C in a fridge short term in a concentrated dilution, This antibody needs to be stored at + 4°
PropertiesC, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by proscience they should be stored frozen at - 24°
Gene target1], Granzyme B [B18
Short name1], anti-Granzyme B Antibody [B18
Technique antibodies against human proteins, antibodies for, antibody to, Antibody
Alternative name1], antibody to-Granzyme B (Antibody to) [B18
Alternative techniqueantibodies

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