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Anti-His Tag Antibody

Anti-His Tag Antibody size: 0.4 ml 406

Supplier NJS POLY
Price 406
Size 0.4 ml
CategoryAntibody
Concentration pH 7, with 0, 09% sodium azide, 4, In 1X PBS
FormPurified
Conjugationhistidine
ClonePolyclonal antibody
Recognised antigenAnti-His Tag
Host animalRabbit (Oryctolagus cuniculus)
ClonalityPolyclonal (rabbit origin)
Species reactivity Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur
Tested applications ELISA, WB
Recommended dilutionsWestern blot: 1:1000
NotesTitration of the anti-His Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity
Intented useThis anti-His Tag antibodyis to be used only for research purposes and not for diagnostics
PurityPurified
Description 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin, Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems
ImmunogenHHHHHH peptide was used as the immunogen for the anti-His Tag antibody
Storage Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the anti-His Tag antibody and store frozen at -20 deg
PropertiesC, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°
Gene targetHis Tag
Short nameAnti-His Tag Antibody
Technique Tags, antibodies against human proteins, antibodies for, Antibody
LabelHis
Alternative nameantibody to-histidine detection labelled (Antibody to)
Alternative techniqueantibodies

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