Name : Anti-His Tag Antibody
Supplier : NJS POLY
Price :406
SKU : GEN8064720032
| Category | Antibody |
| Concentration | pH 7, with 0, 09% sodium azide, 4, In 1X PBS |
| Form | Purified |
| Conjugation | histidine |
| Clone | Polyclonal antibody |
| Recognised antigen | Anti-His Tag |
| Host animal | Rabbit (Oryctolagus cuniculus) |
| Clonality | Polyclonal (rabbit origin) |
| Species reactivity | Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur |
| Tested applications | ELISA, WB |
| Recommended dilutions | Western blot: 1:1000 |
| Notes | Titration of the anti-His Tag antibody may be required due to differences in protocols and secondary/substrate sensitivity |
| Intented use | This anti-His Tag antibodyis to be used only for research purposes and not for diagnostics |
| Purity | Purified |
| Description | 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin, Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems |
| Immunogen | HHHHHH peptide was used as the immunogen for the anti-His Tag antibody |
| Storage | Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the anti-His Tag antibody and store frozen at -20 deg |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target | His Tag |
| Short name | Anti-His Tag Antibody |
| Technique | Tags, antibodies against human proteins, antibodies for, Antibody |
| Label | His |
| Alternative name | antibody to-histidine detection labelled (Antibody to) |
| Alternative technique | antibodies |