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Anti-Insulin Receptor (Polyclonal), ALEXA Fluor 594

Anti-Insulin Receptor (Polyclonal), ALEXA Fluor 594 size: 100 microliters 489

Supplier BIOSS POLYCLONAL ANTIBODIES
Price 489
Size 100 microliters
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideInsulin Receptor
SpecificityThis antibody reacts specifically with Insulin Receptor
ModificationNo modification has been applied to this antibody
Modification siteNone
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsExtracellular
Antigen SourceKLH conjugated synthetic peptide derived from human Insulin Receptor
Gene ID3643
Swiss ProtP06213
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information 2, 3, 4, 4), 5)-triphosphate (PIP3), AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 thereby activating mTORC1 pathway, Another pathway regulated by PI3K-AKT/PKB activation is mTORC1 signaling pathway which regulates cell growth and metabolism and integrates signals from insulin, Binding of insulin leads to phosphorylation of several intracellular substrates, Binding of the SH2 domains of PI3K to phosphotyrosines on IRS1 leads to the activation of PI3K and the generation of phosphatidylinositol-(3, CBL and other signaling intermediates, Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src-homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosines residues, GAB1, Moreover, Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway, SHC, The net effect of this pathway is to produce a translocation of the glucose transporter SLC2A4/GLUT4 from cytoplasmic vesicles to the cell membrane to facilitate glucose transport, a lipid second messenger, activated AKT/PKB is responsible for: anti-apoptotic effect of insulin by inducing phosphorylation of BAD, and the Ras-MAPK pathway, including, including the p85 regulatory subunit of PI3K and SHP2, insulin receptor substrates (IRS1, regulates the expression of gluconeogenic and lipogenic enzymes by controlling the activity of the winged helix or forkhead (FOX) class of transcription factors, such as PDPK1 and subsequently AKT/PKB, upon insulin stimulation, which activates several PIP3-dependent serine/threonine kinases, which is responsible for most of the metabolic actions of insulin, which regulates expression of some genes and cooperates with the PI3K pathway to control cell growth and differentiation, Receptor tyrosine kinase which mediates the pleiotropic actions of insulin
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Synonyms CD220
Also known asInsulin Receptor Polyclonal Antibody
Other nameAnti-Insulin Receptor Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetInsulin Receptor
Short nameAnti-Insulin Receptor (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-Insulin Receptor (polyclonal)
Alternative techniquepolyclonals
Identity 6091
Gene INSR
Long gene name insulin receptor
Locus 19p13, 2
Discovery year 1986-01-01
GenBank acession M10051
Entrez gene record 3643
Pubmed identfication 2983222
Classification CD molecules Fibronectin type III domain containing Receptor Tyrosine Kinases
Havana BLAST/BLAT OTTHUMG00000181992

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