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Anti-MAD1 (Polyclonal), ALEXA Fluor 594

Anti-MAD1 (Polyclonal), ALEXA Fluor 594 size: 100 microliters 489

Price 489
Size 100 microliters
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideMAD1
SpecificityThis antibody reacts specifically with MAD1
ModificationNo modification has been applied to this antibody
Modification siteNone
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsN/A
Antigen SourceKLH conjugated synthetic peptide derived from human MAD1
Gene ID8379
Swiss ProtN/A
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information Like other spindle checkpoint mutants, MAD1 loss-of-function mutants are sensitive to benomyl and cannot delay cell division in response to spindle depolymerization, Mad1p becomes hyperphosphorylated upon spindle depolymerization, The checkpoint works by inhibiting the activity of the anaphase promoting complex, thereby preventing the degradation of several cell cycle regulators, spindle microtubules
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Synonyms MAD1 bHLHc58
Also known asMAD1 Polyclonal Antibody
Other nameAnti-MAD1 Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetMAD1
Short nameAnti-MAD1 (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-MAD1 (polyclonal)
Alternative techniquepolyclonals
Identity 6761
Gene MXD1
Long gene name MAX dimerization protein 1
Synonyms gene MAD
Locus 2p13, 3
Discovery year 1994-02-16
Entrez gene record 4084
Pubmed identfication 7829091
RefSeq identity NM_002357
Classification Basic helix-loop-helix proteins MAX dimerization proteins
Havana BLAST/BLAT OTTHUMG00000129646

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