Name : Anti-MARCH5 (Polyclonal), ALEXA Fluor 594
Supplier : BIOSS POLYCLONAL ANTIBODIES
Price :489
SKU : GEN1453858208
| Type | Conjugated Primary Antibody |
| Conjugated with | 594, ALEXA FLUOR® |
| Host organism | Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide | MARCH5 |
| Specificity | This antibody reacts specifically with 38412 |
| Modification | No modification has been applied to this antibody |
| Modification site | None |
| Clonality | Polyclonal Antibody |
| Clone | Polyclonal Antibodies |
| Concentration | 1ug per 1ul |
| Subcellular locations | N/A |
| Antigen Source | KLH conjugated synthetic peptide derived from human MARCH5 |
| Gene ID | 54708 |
| Swiss Prot | N/A |
| Applications | IF(IHC-P) |
| Applications with corresponding dilutions | IF(IHC-P)(1:50-200) |
| Cross reactive species | Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details | However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information | E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates, MARCH5 has been shown to interact with MTFN2 and ubiquitinated forms of DRP1, This mitochonrial E3 ubiquitin-protein ligase plays an importnat role in controlling the morphology of the mitochondria, MARCH5 is a member of the E3 ubiquitin-protein ligase family |
| Purification method | Purified by Protein A |
| Storage | 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission | 590nm/617nm |
| Synonyms | FLJ20445 MARCH-V MITOL |
| Also known as | MARCH5 Polyclonal Antibody |
| Other name | Anti-MARCH5 Polyclonal |
| Advisory | For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties | For facs or microscopy Alexa 1 conjugate |
| Conjugation | Alexa Fluor |
| Group | Polyclonals and antibodies |
| About | The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target | MARCH5 |
| Short name | Anti-MARCH5 (Polyclonal) Fluor 594 |
| Technique | Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different  |
| Label | ALEXA |
| Alternative name | ALEXA Fluor 594, antibody to-MARCH5 (polyclonal) |
| Alternative technique | polyclonals |
| Identity | 26025 |
| Gene | MARCH5 |
| Long gene name | membrane associated ring-CH-type finger 5 |
| Synonyms gene | RNF153 |
| Synonyms gene name | ring finger protein 153 membrane-associated ring finger (C3HC4) 5 |
| Synonyms name | mitochondrial ubiquitin ligase |
| Locus | 10q23, 32-q23, 33 |
| Discovery year | 2004-05-27 |
| GenBank acession | BC015480 |
| Entrez gene record | 54708 |
| Pubmed identfication | 14722266 |
| RefSeq identity | NM_017824 |
| Classification | Membrane associated ring-CH-type fingers Ring finger proteins |
| Havana BLAST/BLAT | OTTHUMG00000018757 |