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Anti-MBD1 (Polyclonal), ALEXA Fluor 594

Anti-MBD1 (Polyclonal), ALEXA Fluor 594 size: 100 microliters 489

Supplier BIOSS POLYCLONAL ANTIBODIES
Price 489
Size 100 microliters
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideMBD1
SpecificityThis antibody reacts specifically with MBD1
ModificationNo modification has been applied to this antibody
Modification siteNone
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsN/A
Antigen SourceKLH conjugated synthetic peptide derived from human MBD1
Gene ID4152
Swiss ProtN/A
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract, MBD1, MBD2, MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal, MBD3, MBD3 does not bind methylated DNA both in vivo and in vitro, MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases, MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA, Surprisingly, The demethylation enzyme was recently found to be a mammalian protein, The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol, The methylation pattern involves the enzymatic processes of methylation and demethylation, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, is imperative to proper development and regulates gene expression, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, which are known to be deficient in MeCP1 activity, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD), DNA methylation
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Synonyms PCM1 CXXC3
Also known asMBD1 Polyclonal Antibody
Other nameAnti-MBD1 Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetMBD1
Short nameAnti-MBD1 (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-MBD1 (polyclonal)
Alternative techniquepolyclonals
Identity 6916
Gene MBD1
Long gene name methyl-CpG binding domain protein 1
Locus 18q21, 1
Discovery year 1997-10-29
GenBank acession Y10746
Entrez gene record 4152
Pubmed identfication 9207790 10441743
RefSeq identity NM_015846
Classification Methyl-CpG binding domain containing Zinc fingers CXXC-type
Havana BLAST/BLAT OTTHUMG00000132669

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