Name : Anti-NCKAP1 (Polyclonal), ALEXA Fluor 594
Supplier : BIOSS POLYCLONAL ANTIBODIES
Price :489
SKU : GEN6267664834
| Type | Conjugated Primary Antibody |
| Conjugated with | 594, ALEXA FLUOR® |
| Host organism | Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide | NCKAP1 |
| Specificity | This antibody reacts specifically with NCKAP1 |
| Modification | No modification has been applied to this antibody |
| Modification site | None |
| Clonality | Polyclonal Antibody |
| Clone | Polyclonal Antibodies |
| Concentration | 1ug per 1ul |
| Subcellular locations | N/A |
| Antigen Source | KLH conjugated synthetic peptide derived from human 12 NCKAP1 |
| Gene ID | 10787 |
| Swiss Prot | N/A |
| Applications | IF(IHC-P) |
| Applications with corresponding dilutions | IF(IHC-P)(1:50-200) |
| Cross reactive species | Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details | However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information | Abi-1 and CYFIP1, NAP125 expression is markedly reduced in Alzheimer disease (AD)-affected brains, NAP125 is found at highest levels in heart, NAP125 localizes to the cytoplasmic side of lamellipodium membrane and is encoded by a gene that maps to human chromosome 2q32, While widely expressed, also known as NCKAP1 (NCK-associated protein 1), brain and skeletal muscle where it regulates Rac-dependent actin remodeling as part of a lamellipodial complex with WAVE2, is a 1, p125Nap1 or membrane-associated protein HEM-2, suggesting a possible role in the disease, 1 and mouse chromosome 2 C3, 128 amino acid single pass membrane protein that exists as two alternatively spliced isoforms and belongs to the HEM-1/HEM-2 family, NAP125 |
| Purification method | Purified by Protein A |
| Storage | 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission | 590nm/617nm |
| Synonyms | Nap1 HEM2 NAP125 |
| Also known as | NCKAP1 Polyclonal Antibody |
| Other name | Anti-NCKAP1 Polyclonal |
| Advisory | For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties | For facs or microscopy Alexa 1 conjugate |
| Conjugation | Alexa Fluor |
| Group | Polyclonals and antibodies |
| About | The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target | NCKAP1 |
| Short name | Anti-NCKAP1 (Polyclonal) Fluor 594 |
| Technique | Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different  |
| Label | ALEXA |
| Alternative name | ALEXA Fluor 594, antibody to-NCK-associated protein 1 (polyclonal) |
| Alternative technique | polyclonals |
| Alternative to gene target | HEM2 and NAP1 and NAP125 and p125Nap1, NCKAP1 and IDBG-639791 and ENSBTAG00000033662 and 100300817, NCKAP1 and IDBG-76903 and ENSG00000061676 and 10787, Nckap1 and IDBG-183835 and ENSMUSG00000027002 and 50884, Plasma membranes, Rac GTPase binding, embryo and biological process this GO :0007417 and central nervous system development and biological process this GO :0007492 and endoderm development and biological process this GO :0008078 and mesodermal cell migration and biological process this GO :0010172 and embryonic body morphogenesis and biological process this GO :0010592 and positive regulation of lamellipodium assembly and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0016601 and Rac protein signal transduction and biological process this GO :0030027 and lamellipodium and cellular component this GO :0030032 and lamellipodium assembly and biological process this GO :0030903 and notochord development and biological process this GO :0030950 and establishment or maintenance of actin cytoskeleton polarity and biological process this GO :0031209 and SCAR complex and cellular component this GO :0031258 and lamellipodium membrane and cellular component this GO :0032403 and protein complex binding and molecular function this GO :0032880 and regulation of protein localization and biological process this GO :0035050 and embryonic heart tube development and biological process this GO :0038096 and Fc-gamma receptor signaling pathway involved in pha this GO cytosis and biological process this GO :0042074 and cell migration involved in gastrulation and biological process this GO :0045087 and innate immune response and biological process this GO :0045175 and basal protein localization and biological process this GO :0045176 and apical protein localization and biological process this GO :0048339 and paraxial mesoderm development and biological process this GO :0048340 and paraxial mesoderm morphogenesis and biological process this GO :0048365 and Rac GTPase binding and molecular function this GO :0048570 and notochord morphogenesis and biological process this GO :0048617 and embryonic foregut morphogenesis and biological process this GO :0050821 and protein stabilization and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :2000601 and positive regulation of Arp2/3 complex-mediated actin nucleation and biological process, this GO :0001701 and in utero embryonic development and biological process this GO :0001756 and somitogenesis and biological process this GO :0001843 and neural tube closure and biological process this GO :0005515 and protein binding and molecular function this GO :0005829 and cytosol and cellular component this GO :0006915 and apoptotic process and biological process this GO :0007354 and zy this GO tic determination of anterior/posterior axis, this GO :0005515: protein binding, this GO :0005515: protein binding and also this GO :0032403: protein complex binding and also this GO :0048365: Rac GTPase binding, this GO :0032403: protein complex binding, this GO :0048365: Rac GTPase binding, 101907541, NCK-associated protein 1 |
| Identity | 7666 |
| Gene | NCKAP1 |
| Long gene name | NCK associated protein 1 |
| Locus | 2q32, 1 |
| Discovery year | 1999-07-19 |
| GenBank acession | AB014509 |
| Entrez gene record | 10787 |
| Pubmed identfication | 10673335 12181570 9344857 |
| RefSeq identity | NM_205842 |
| Havana BLAST/BLAT | OTTHUMG00000132623 |