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Anti-NEDD8 (Polyclonal), ALEXA Fluor 594

Anti-NEDD8 (Polyclonal), ALEXA Fluor 594 size: 100 microliters 489

Supplier BIOSS POLYCLONAL ANTIBODIES
Price 489
Size 100 microliters
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideNEDD8
SpecificityThis antibody reacts specifically with NEDD8
ModificationNo modification has been applied to this antibody
Modification siteNone
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsN/A
Antigen SourceKLH conjugated synthetic peptide derived from human NEDD8
Gene ID4738
Swiss ProtN/A
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information Apg12 and Hub1, Cullins are subunits of an E3 ubiquitin ligase complex called the Skp1 /Cul1/Cdc53 F box (SCF), Examples of UBLs are SUMO, In contrast to UBLs, NEDD8 shows 80% homology to ubiquitin, Proteins of the second class include Parkin, RAD23 and DSK2, Rub1 (also called Nedd8), Rub1 and the corresponding human homolog NEDD8 are activated by the E1 ubiquitin activating enzyme UBA2, Rub1 modification may regulate SCF function or localization, The SCF promotes transfer of ubiquitin from a ubiquitin conjugating enzyme (E2) to the target protein, The best known targets of Rub1 modification are members of the cullin family, These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other, UDPs are not proteolytically processed or conjugated to other proteins, are designated ubiquitin domain proteins (UDPs), that forms isopeptide linkages between thio esters, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin, Ubiquitin like proteins fall into two classes: the first class
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Synonyms Nedd-8
Also known asNEDD8 Polyclonal Antibody
Other nameAnti-NEDD8 Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetNEDD8
Short nameAnti-NEDD8 (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-NEDD8 (polyclonal)
Alternative techniquepolyclonals
Identity 7732
Gene NEDD8
Long gene name developmentally down-regulated 8 , neural precursor cell expressed
Locus 14q12
Discovery year 1994-01-21
GenBank acession D23662
Entrez gene record 4738
Pubmed identfication 9353319
RefSeq identity NM_006156
Havana BLAST/BLAT OTTHUMG00000029325

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