Anti-NFKB p65 (Polyclonal), Cy3

Anti-NFKB p65 (Polyclonal), Cy3 size: 100 microliters 516

Price 516

Size 100 microliters

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Type	Conjugated Primary Antibody
Conjugated with	Cy3
Host organism	Rabbit (Oryctolagus cuniculus)
Target Protein/Peptide	NFKB p65
Specificity	This antibody reacts specifically with NFKB p65 Cy3
Modification	No modification has been applied to this antibody
Modification site	None
Clonality	Polyclonal Antibody
Clone	Polyclonal Antibodies
Concentration	1ug per 1ul
Subcellular locations	Nucleus, Cytoplasm
Antigen Source	KLH conjugated synthetic peptide derived from human NFKB p65
Gene ID	5970
Swiss Prot	Q04206
Applications	IF(IHC-P)
Applications with corresponding dilutions	IF(IHC-P)(1:50-200)
Cross reactive species	Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details	However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information	Associates with chromatin at the NF-kappa-B promoter region via association with DDX1, Different dimer combinations act as transcriptional activators or repressors, Essential for cytokine gene expression in T-cells (PubMed:15790681), I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, In a conventional activation pathway, NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family, NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators, NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one, RELB, The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression, The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity, The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65, cell growth, differentiation, immunity, p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex, respectively, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus, tumorigenesis and apoptosis, NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation
Purification method	Purified by Protein A
Storage	50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission	512, 550nm/570, 615nm
Synonyms	NFKB3, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, Transcription factor p65, p65
Also known as	NFKB p65 Polyclonal Antibody Cy3
Other name	Anti-NFKB p65 Polyclonal Cy3
Advisory	For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
Properties	1 of the Anti-NFKB p65 (Polyclonal) can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC, This Bioss Polyclonal Antibodies antibody is well suited for fluorescent microscopy, Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser
Conjugation	cy3 conjugation kit
Group	Polyclonals and antibodies
About	The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene target	NFKB p65
Short name	Anti-NFKB p65 (Polyclonal)
Technique	Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different
Label	Cy3
Alternative name	cyanine 3, antibody to-NFKB p65 (polyclonal)
Alternative technique	polyclonals

Anti-NFKB p65 (Polyclonal), Cy3

Anti-NFKB p65 (Polyclonal), Cy3 size: 100 microliters 516

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