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Anti-Nidogen 2 (Polyclonal), ALEXA Fluor 594

Anti-Nidogen 2 (Polyclonal), ALEXA Fluor 594 size: 100 microliters 489

Price 489
Size 100 microliters
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideNidogen 2
SpecificityThis antibody reacts specifically with Nidogen 2
ModificationNo modification has been applied to this antibody
Modification siteNone
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locations Secreted, Extracellular
Antigen SourceKLH conjugated synthetic peptide derived from human Nidogen 2
Gene ID22795
Swiss ProtN/A
Applications IF(IHC-P), FCM
Applications with corresponding dilutions IF(IHC-P)(1:50-200), FCM(1:20-100)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information IV and Perlecan, In mammals, Nidogen and Nidogen-2, Nidogen-2 is a cell adhesion protein glycosylated at nitrogen and oxygen sites, Nidogens connect the Laminin and Collagen IV networks and integrate other proteins into the membrane, The two isoforms have a similar shape, and co-localize in vessel walls and other basement membrane zones, and is widely distributed in basement membranes in heart, bone and, consisting of three globular domains, in pancreas, interact at comparable levels with Collagen I, kidney and skeletal muscle, placenta, serving to stabilize basement membranes and playing a major role in embryogenesis, to a lesser extent, two Nidogen proteins, Nidogens are highly conserved proteins present in vertebrate and invertebrate basement membranes
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Also known asNidogen 2 Polyclonal Antibody
Other nameAnti-Nidogen 2 Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetNidogen 2
Short nameAnti-Nidogen 2 (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-Nidogen 2 (polyclonal)
Alternative techniquepolyclonals
Identity 13389
Gene NID2
Long gene name nidogen 2
Synonyms name osteonidogen
Locus 14q22, 1
Discovery year 2001-06-25
GenBank acession AB009799
Entrez gene record 22795
Pubmed identfication 9733643
Havana BLAST/BLAT OTTHUMG00000140298

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