Name : Anti-p73(Tyr99) (Polyclonal), ALEXA Fluor 594
Supplier : BIOSS POLYCLONAL ANTIBODIES
Price :516
SKU : GEN3459673904
| Type | Conjugated Primary Antibody |
| Conjugated with | 594, ALEXA FLUOR® |
| Host organism | Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide | p73 Tyr99 |
| Specificity | This antibody reacts specifically with p73(Tyr99) |
| Modification | Phosphorylation |
| Modification site | Tyr99 |
| Clonality | Polyclonal Antibody |
| Clone | Polyclonal Antibodies |
| Concentration | 1ug per 1ul |
| Subcellular locations | N/A |
| Antigen Source | KLH conjugated synthetic phosphopeptide derived from human p73 around the phosphorylation site of Tyr99 [SP(p-Y)AQ] |
| Gene ID | 7161 |
| Swiss Prot | N/A |
| Applications | IF(IHC-P) |
| Applications with corresponding dilutions | IF(IHC-P)(1:50-200) |
| Cross reactive species | Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details | However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information | Alternative splicing of the proto-oncogene H-ras pre-mRNA has led to two distinct transcripts, However, In this study, In this study, Oncogenic MDM2 (mouse double minutes 2) is a known repressor of p73 transcriptional activity, Ras proteins are known to be small membrane-localized guanine nucleotide-binding proteins, The p73 protein, Therefore, a tumor suppressor gene, by the yeast two-hybrid screening method, involving p19ras and p73, it further inhibited the association of MDM2 to the p73 protein, p19ras, p19ras is localized in the nucleus and the cytosol and its interaction with P73 protein occurred exclusively in the nucleus, this study presents a novel pathway of Ras signaling that occurs in the nucleus, unlike other Ras proteins, when p19ras was bound to MDM2, P73 protein is a structural and functional homologue of p53 |
| Purification method | Purified by Protein A |
| Storage | 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission | 590nm/617nm |
| Synonyms | P73_HUMAN, TP 73, TP73, TP73phospho Y99, Tumor Protein p73, p53 Related Protein, p53 like transcription factor, p73, p73Tyr99 |
| Also known as | p73(Tyr99) Polyclonal Antibody |
| Other name | Anti-p73(Tyr99) Polyclonal |
| Advisory | For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties | For facs or microscopy Alexa 1 conjugate |
| Conjugation | Alexa Fluor |
| Group | Polyclonals and antibodies |
| About | The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target | p73(Tyr99) |
| Short name | Anti-p73(Tyr99) (Polyclonal) Fluor 594 |
| Technique | Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different  |
| Label | ALEXA |
| Alternative name | ALEXA Fluor 594, antibody to-p73(Tyr99) (polyclonal) |
| Alternative technique | polyclonals |