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Anti-PAK1(Thr212) (Polyclonal), ALEXA Fluor 594
Anti-PAK1(Thr212) (Polyclonal), ALEXA Fluor 594 size: 100 microliters 516
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BIOSS POLYCLONAL ANTIBODIES
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516
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100 microliters
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| Type | Conjugated Primary Antibody |
| Conjugated with | 594, ALEXA FLUOR® |
| Host organism | Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide | PAK1 Thr212 |
| Specificity | This antibody reacts specifically with PAK1(Thr212) |
| Modification | Phosphorylation |
| Modification site | Thr212 |
| Clonality | Polyclonal Antibody |
| Clone | Polyclonal Antibodies |
| Concentration | 1ug per 1ul |
| Subcellular locations | N/A |
| Antigen Source | KLH conjugated synthetic phosphopeptide derived from human PAK1 around the phosphorylation site of Thr212 |
| Gene ID | 5058 |
| Swiss Prot | N/A |
| Applications | IF(IHC-P) |
| Applications with corresponding dilutions | IF(IHC-P)(1:50-200) |
| Cross reactive species | Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details | However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information | CDC42 and RAC1, The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and have been implicated in a wide range of biological activities, and may play a role in regulating the apoptotic events in the dying cell, The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling |
| Purification method | Purified by Protein A |
| Storage | 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission | 590nm/617nm |
| Also known as | PAK1(Thr212) Polyclonal Antibody |
| Other name | Anti-PAK1(Thr212) Polyclonal |
| Advisory | For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties | For facs or microscopy Alexa 1 conjugate |
| Conjugation | Alexa Fluor |
| Group | Polyclonals and antibodies |
| About | The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target | PAK1(Thr212) |
| Short name | Anti-PAK1(Thr212) (Polyclonal) Fluor 594 |
| Technique | Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label | ALEXA |
| Alternative name | ALEXA Fluor 594, antibody to-p21 protein (Cdc42/Rac)-activated kinase 1(Thr212) (polyclonal) |
| Alternative technique | polyclonals |
| Alternative to gene target | PAK1 and IDBG-642220 and ENSBTAG00000010191 and 533729, PAK1 and IDBG-65610 and ENSG00000149269 and 5058, PAKalpha, Pak1 and IDBG-199974 and ENSMUSG00000030774 and 18479, nuclei, this GO :0001666 and response to hypoxia and biological process this GO :0001726 and ruffle and cellular component this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005518 and collagen binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005911 and cell-cell junction and cellular component this GO :0005925 and focal adhesion and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0006887 and exocytosis and biological process this GO :0006915 and apoptotic process and biological process this GO :0007015 and actin filament organization and biological process this GO :0007411 and axon guidance and biological process this GO :0007528 and neuromuscular junction development and biological process this GO :0010033 and response to organic substance and biological process this GO :0014704 and intercalated disc and cellular component this GO :0016020 and membrane and cellular component this GO :0016358 and dendrite development and biological process this GO :0016772 and transferase activity, this GO :0004672: protein kinase activity, this GO :0004672: protein kinase activity and also this GO :0004674: protein serine/threonine kinase activity and also this GO :0004713: protein tyrosine kinase activity and also this GO :0005515: protein binding and also this GO :0005518: collagen binding and also this GO :0005524: ATP binding and also this GO :0016772: transferase activity, this GO :0004674: protein serine/threonine kinase activity, this GO :0004713: protein tyrosine kinase activity, this GO :0005515: protein binding, this GO :0005518: collagen binding, this GO :0005524: ATP binding, this GO :0016772: transferase activity, this GO :0019901: protein kinase binding, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0019901: protein kinase binding, transferring phosphorus-containing groups and molecular function this GO :0019901 and protein kinase binding and molecular function this GO :0023014 and signal transduction by phosphorylation and biological process this GO :0030018 and Z disc and cellular component this GO :0030424 and axon and cellular component this GO :0030425 and dendrite and cellular component this GO :0030426 and growth cone and cellular component this GO :0031295 and T cell costimulation and biological process this GO :0031532 and actin cytoskeleton reorganization and biological process this GO :0031941 and filamentous actin and cellular component this GO :0031965 and nuclear membrane and cellular component this GO :0032587 and ruffle membrane and cellular component this GO :0032869 and cellular response to insulin stimulus and biological process this GO :0032956 and regulation of actin cytoskeleton organization and biological process this GO :0033138 and positive regulation of peptidyl-serine phosphorylation and biological process this GO :0033148 and positive regulation of intracellular estrogen receptor signaling pathway and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0038096 and Fc-gamma receptor signaling pathway involved in pha this GO cytosis and biological process this GO :0042060 and wound healing and biological process this GO :0043113 and receptor clustering and biological process this GO :0043234 and protein complex and cellular component this GO :0043507 and positive regulation of JUN kinase activity and biological process this GO :0045087 and innate immune response and biological process this GO :0046777 and protein autophosphorylation and biological process this GO :0048754 and branching morphogenesis of an epithelial tube and biological process this GO :0048812 and neuron projection morphogenesis and biological process this GO :0050852 and T cell receptor signaling pathway and biological process this GO :0051496 and positive regulation of stress fiber assembly and biological process this GO :0060244 and negative regulation of cell proliferation involved in contact inhibition and biological process, p21 protein (Cdc42/Rac)-activated kinase 1 |
| Identity | 8590 |
| Gene | PAK1 |
| Long gene name | p21 (RAC1) activated kinase 1 |
| Synonyms gene name | p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related) p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) p21 protein (Cdc42/Rac)-activated kinase 1 |
| Synonyms name | STE20 homolog, yeast |
| Locus | 11q13, 1 , 5-q14 |
| Discovery year | 1997-12-05 |
| GenBank acession | U51120 |
| Entrez gene record | 5058 |
| Pubmed identfication | 8805275 9533029 |
| RefSeq identity | NM_002576 |
| Havana BLAST/BLAT | OTTHUMG00000165138 |