Name : anti-Perforin Antibody [CB5.4]
Supplier : PROSCIENCE
Price :180
SKU : GEN9558187738
| Gene names | Cytolysin, P1, Perforin-1, Pfn, Pfp, Prf-1, Pfp |
| Antibodie's label | unlabelled serum |
| Purity | #8805, 4) antibody was purified to &, 95% in SDS-PAGE, Perforin (monoclonal CB5 |
| Ab raised in | Rat |
| Type | Monoclonal |
| Monoclonal | 4, CB5 |
| Immunoglobulin | IgG2a |
| Antigen | amino acids 98-534, 4) antibody was made against a recombinant mouse perforin, Perforin (monoclonal CB5 |
| Immuno application | immuno precipitation, immuno-cyto-chemistry, immuno-histo-chemistry, Western Blot |
| Antibody advice | 0, After 3 washings in PBS, Method: A mouse T cell clone was plated onto polylysine treated glass slides, Method: Cell extracts from the T cell clone B6, Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system, This product can be used for detection of active human FasL by flow cytometry and western blot, and developed in appropriate substrate solution (DAB or AEC), fixed and permeabilized in methanol at -20oC for 5 min, incubated in anti-rat IgG-HRP, slides were incubated with 5 ug/mL of CB5, then in acetone at -20oC for 30 sec, transferred to nitrocellulose and probed with the CB5, washed in PBS, washed in PBS, 1 (2x106) were resolved by SDS-PAGE under reducing conditions, 1% BSA, 4 antibody, 4 antibody at 1 ug/mL, Perforin antibody recognizes only membrane bound active (non-cleaved) human FasL and not the inactive soluble FasL |
| Suspention, pH, azide | 02% sodium azide, PBS containing 0 |
| Keep at | stable for one year, Perforin antibody can be stored at 4 grades C |
| Omim nr | 129820 |
| Ncbi nr | P10820 |
| Vial with antibody | in solution |
| NCBI GENE nr | 18646 |
| GENE symbol | Prf1 |
| Antigene name | perforin 1 (pore forming protein) |
| Source | Mus musculus |
| Protein nr | P10820 |
| Description | Freeze thaw will destroy a percentage in every cycle and should be avoided, C in a fridge short term in a concentrated dilution, This antibody needs to be stored at + 4° |
| Properties | C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by proscience they should be stored frozen at - 24° |
| Gene target | 4], Perforin [CB5 |
| Short name | 4], anti-Perforin Antibody [CB5 |
| Technique | antibodies against human proteins, antibodies for, antibody to, Antibody |
| Alternative name | 4], antibody to-Perforin (Antibody to) [CB5 |
| Alternative technique | antibodies |