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Anti-PIM1(Tyr309) (Polyclonal), ALEXA Fluor 594
Anti-PIM1(Tyr309) (Polyclonal), ALEXA Fluor 594 size: 100 microliters 516
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BIOSS POLYCLONAL ANTIBODIES
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516
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100 microliters
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| Type | Conjugated Primary Antibody |
| Conjugated with | 594, ALEXA FLUOR® |
| Host organism | Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide | PIM1 Tyr309 |
| Specificity | This phosphorylation site is homologous to Tyr302 in Mouse and Tyr218 in Rat, as this is the position in Isoform 2 of this protein, PIM1(Tyr309), This phosphorylation site is also referred to as Tyr218 |
| Modification | Phosphorylation |
| Modification site | Tyr309 |
| Clonality | Polyclonal Antibody |
| Clone | Polyclonal Antibodies |
| Concentration | 1ug per 1ul |
| Subcellular locations | Helical |
| Antigen Source | KLH conjugated synthetic phosphopeptide derived from human PIM1 around the phosphorylation site of Tyr218 |
| Gene ID | 5292 |
| Swiss Prot | P11309 |
| Applications | IF(IHC-P) |
| Applications with corresponding dilutions | IF(IHC-P)(1:50-200) |
| Cross reactive species | Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details | However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information | Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis, CDKN1B, Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, FOXO3), MAP3K5, May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3, Mediates survival signaling through phosphorylation of BAD, Phosphorylation of CDKN1A, Phosphorylation of CDKN1B, Phosphorylation of MAP3K5, Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity, Promote cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C, The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis, a regulator of cell cycle progression at G1, an other proapoptotic protein, at both transcriptional and post-translational levels, by PIM1, nuclear export and proteasome-dependent degradation, results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability, significantly decreases MAP3K5 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1, Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis, induces 14-3-3-proteins binding |
| Purification method | Purified by Protein A |
| Storage | 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission | 590nm/617nm |
| Also known as | PIM1(Tyr309) Polyclonal Antibody |
| Other name | Anti-PIM1(Tyr309) Polyclonal |
| Advisory | For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties | For facs or microscopy Alexa 1 conjugate |
| Conjugation | Alexa Fluor |
| Group | Polyclonals and antibodies |
| About | The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target | PIM1(Tyr309) |
| Short name | Anti-PIM1(Tyr309) (Polyclonal) Fluor 594 |
| Technique | Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label | ALEXA |
| Alternative name | ALEXA Fluor 594, antibody to-pim-1 oncogene(Tyr309) (polyclonal) |
| Alternative technique | polyclonals |
| Alternative to gene target | PIM, PIM1 and IDBG-630304 and ENSBTAG00000000396 and 281402, PIM1 and IDBG-85488 and ENSG00000137193 and 5292, Pim1 and IDBG-160725 and ENSMUSG00000024014 and 18712, alcohol group as acceptor, alcohol group as acceptor and also this GO :0030145: manganese ion binding and also this GO :0043024: ribosomal small subunit binding, alcohol group as acceptor and molecular function this GO :0030145 and manganese ion binding and molecular function this GO :0030212 and hyaluronan metabolic process and biological process this GO :0031659 and positive regulation of cyclin-dependent protein kinase activity involved in G1/S and biological process this GO :0043024 and ribosomal small subunit binding and molecular function this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0043433 and negative regulation of sequence-specific DNA binding transcription factor activity and biological process this GO :0046777 and protein autophosphorylation and biological process, nuclei, this GO :0004672 and protein kinase activity and molecular function this GO :0004674 and protein serine/threonine kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0006915 and apoptotic process and biological process this GO :0007049 and cell cycle and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0008283 and cell proliferation and biological process this GO :0009103 and lipopolysaccharide biosynthetic process and biological process this GO :0016020 and membrane and cellular component this GO :0016772 and transferase activity, this GO :0004672: protein kinase activity, this GO :0004672: protein kinase activity and also this GO :0004674: protein serine/threonine kinase activity and also this GO :0004713: protein tyrosine kinase activity and also this GO :0005515: protein binding and also this GO :0005524: ATP binding and also this GO :0008134: transcription factor binding and also this GO :0016772: transferase activity, this GO :0004674: protein serine/threonine kinase activity, this GO :0004713: protein tyrosine kinase activity, this GO :0005515: protein binding, this GO :0005524: ATP binding, this GO :0008134: transcription factor binding, this GO :0016772: transferase activity, this GO :0016773: phosphotransferase activity, this GO :0030145: manganese ion binding, this GO :0043024: ribosomal small subunit binding, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0016773: phosphotransferase activity, transferring phosphorus-containing groups and molecular function this GO :0016773 and phosphotransferase activity, pim-1 oncogene |
| Identity | 8986 |
| Gene | PIM1 |
| Long gene name | Pim-1 proto-oncogene, serine/threonine kinase |
| Synonyms gene | PIM |
| Synonyms gene name | pim-1 oncogene |
| Locus | 6p21, 2 |
| Discovery year | 1986-01-01 |
| Entrez gene record | 5292 |
| Havana BLAST/BLAT | OTTHUMG00000016426 |