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Anti-RNA polymerase II (Polyclonal), ALEXA Fluor 594

Anti-RNA polymerase II (Polyclonal), ALEXA Fluor 594 size: 100 microliters 489

Supplier BIOSS POLYCLONAL ANTIBODIES
Price 489
Size 100 microliters
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideRNA polymerase II
SpecificityThis antibody reacts specifically with RNA polymerase II
ModificationNo modification has been applied to this antibody
Modification siteNone
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsN/A
Antigen SourceKLH conjugated synthetic peptide derived from human Pol II/RNA polymerase II
Gene IDN/A
Swiss ProtN/A
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information Additionally, Although Pol II function requires the cooperation of all twelve subunits, The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1, Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, Without proper function of the large subunit, a groove that is necessary for transcription of the DNA template, forms the active center of the Pol II enzyme, mRNA synthesis and subsequent transcription elongation cannot occur, the large subunit participates in forming the DNA-binding domain of Pol II, the largest subunit conveys Pol II catalytic activity and, thereby allowing transcription to begin, together with the second largest subunit, 840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats, RNA polymerase II (Pol II) is an enzyme that is composed of twelve subunits and is responsible for the transcription of protein-coding genes
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Synonyms RBP4
Also known asRNA polymerase II Polyclonal Antibody
Other nameAnti-RNA polymerase II Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetRNA polymerase II
Short nameAnti-RNA polymerase II (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-RNA polymerizing enzyme II (polyclonal)
Alternative techniquepolyclonals
Identity 9191
Gene POLR2D
Long gene name RNA polymerase II subunit D
Synonyms gene name polymerase (RNA) II (DNA directed) polypeptide D
Synonyms name RNA polymerase II subunit hsRBP4
Locus 2q14, 3
Discovery year 1993-12-07
GenBank acession U85510
Entrez gene record 5433
Pubmed identfication 9528765
RefSeq identity NM_004805
Classification RNA polymerase subunits
Havana BLAST/BLAT OTTHUMG00000131531

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