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Anti-RNF25 (Polyclonal), ALEXA Fluor 594

Anti-RNF25 (Polyclonal), ALEXA Fluor 594 size: 100 microliters 489

Price 489
Size 100 microliters
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideRNF25
SpecificityThis antibody reacts specifically with RNF25
ModificationNo modification has been applied to this antibody
Modification siteNone
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsN/A
Antigen SourceKLH conjugated synthetic peptide derived from human RNF25
Gene ID64320
Swiss ProtN/A
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information RNF25 (RING finger protein 25), RNF25 is thought to exhibit E2-dependent E3 ubiquitin-protein ligase activity, RNF25 may also regulate the transcriptional activity of NF-kappa-B, Specifically, The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), Via its RING-type zinc finger (a common domain that contains a conserved cysteine-rich region and is found in a number of viral and eukaryotic proteins), a protein that plays important roles in cell survival, also known as AO7, facilitates the ubiquitination of target proteins, host defense and proliferation, inflammation response, is a 459 amino acid protein that contains one RWD domain and one RING-type zinc finger, the RING finger of RNF25 interacts with an E2 ligase and, the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s), through this interaction, Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Synonyms AO7 FLJ13906
Also known asRNF25 Polyclonal Antibody
Other nameAnti-RNF25 Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetRNF25
Short nameAnti-RNF25 (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-RNF25 (polyclonal)
Alternative techniquepolyclonals
Identity 14662
Gene RNF25
Long gene name ring finger protein 25
Locus 2q35
Discovery year 2001-02-21
Entrez gene record 64320
Pubmed identfication 12748188
RefSeq identity NM_022453
Classification Ring finger proteins
Havana BLAST/BLAT OTTHUMG00000133077

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