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Anti-Smad2(Ser465 + Ser467), ALEXA Fluor 594

Anti-Smad2(Ser465 + Ser467), ALEXA Fluor 594 size: 100 microliters 516

Supplier BIOSS POLYCLONAL ANTIBODIES
Price 516
Size 100 microliters
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideSmad2 Ser465 + Ser467
SpecificityThis antibody reacts specifically with Smad2(Ser465 + Ser467)
ModificationPhosphorylation
Modification siteSer465 + Ser467
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsN/A
Antigen SourceKLH conjugated synthetic phosphopeptide derived from human SMAD2 around the phosphorylation site of Ser465/467
Gene ID4087
Swiss ProtN/A
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information (Smad4) that functions via its interaction to the various Smad's, (Smad6 and Smad7), 5, Activated Smad2 associates with Smad4 and translocates as a complex into the nucleus, Phosphorylation of threonine 8 in the calmodulin binding region of the MH1 domain by extracellular signal regulated kinase 1(ERK 1) enhances Smad2 transcriptional activity, Smad2 consists of two highly conserved domains, The MH1 domain binds DNA and regulates nuclear import and transcription while the MH2 domain conserved among all the Smad's regulates Smad2 oligomerization and binding to cytoplasmic adaptors and transcription factors, The Smad family is divided into three subclasses: receptor-regulated Smad's, The regulation of Smad2 phosphorylation on threonine 8 by ERK 1 and calmodulin is critical for Smad2 mediated signaling, This translocation of Smad2 (as well as Smad3) into the nucleus is a central event in TGF beta signaling, activin/TGF alpha receptor-regulated (Smad2 and 3) or BMP receptor regulated (Smad1, allowing its binding to DNA and transcription factors, and 8), and the inhibitory Smad's, differentiation and development, the N terminal Mad homology (MH1) and the C-terminal Mad homology 2 (MH2) domains, the common partner, which is negatively regulated by calmodulin, Smad2 is a 58 kDa member of a family of proteins involved in cell proliferation
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Synonyms JV18, JV18 1, JV181, MAD, MAD Related Protein 2, MADH2, MADR2, MGC22139, MGC34440, Mothers Against Decapentaplegic Homolog 2, SMAD, SMAD 2, SMAD2, SMAD2_HUMAN, Smad2p-S465/467, hMAD 2, hSMAD2, mothers against DPP homolog 2, p-Smad2Ser465/467, Smad2p-Ser465/467
Also known asSmad2(Ser465 + Ser467) Antibody
Other nameAnti-Smad2(Ser465 + Ser467)
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
Gene targetSmad2(Ser465 + Ser467)
Short nameAnti-Smad2(Ser465 + Ser467) Fluor 594
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-SMAD family member 2(Ser465 and Ser467)

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