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Anti-SPHK1 (Polyclonal), ALEXA Fluor 594
Anti-SPHK1 (Polyclonal), ALEXA Fluor 594 size: 100 microliters 489
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BIOSS POLYCLONAL ANTIBODIES
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489
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100 microliters
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| Type | Conjugated Primary Antibody |
| Conjugated with | 594, ALEXA FLUOR® |
| Host organism | Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide | SPHK1 |
| Specificity | This antibody reacts specifically with SPHK1 |
| Modification | No modification has been applied to this antibody |
| Modification site | None |
| Clonality | Polyclonal Antibody |
| Clone | Polyclonal Antibodies |
| Concentration | 1ug per 1ul |
| Subcellular locations | Nucleus, Cytoplasm |
| Antigen Source | KLH conjugated synthetic peptide derived from human SPHK1 |
| Gene ID | 8877 |
| Swiss Prot | N/A |
| Applications | IF(IHC-P) |
| Applications with corresponding dilutions | IF(IHC-P)(1:50-200) |
| Cross reactive species | Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details | However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information | Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, Intracellularly, MIM 173430) and serum, Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), and extracellularly, including platelet-derived growth factor (e, it is a ligand for EDG1 (MIM 601974), it regulates proliferation and survival, the enzyme that catalyzes the phosphorylation of sphingosine, Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions, [supplied by OMIM], g |
| Purification method | Purified by Protein A |
| Storage | 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission | 590nm/617nm |
| Synonyms | SPHK |
| Also known as | SPHK1 Polyclonal Antibody |
| Other name | Anti-SPHK1 Polyclonal |
| Advisory | For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties | For facs or microscopy Alexa 1 conjugate |
| Conjugation | Alexa Fluor |
| Group | Polyclonals and antibodies |
| About | The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target | SPHK1 |
| Short name | Anti-SPHK1 (Polyclonal) Fluor 594 |
| Technique | Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label | ALEXA |
| Alternative name | ALEXA Fluor 594, antibody to-sphin this GO sine kinase 1 (polyclonal) |
| Alternative technique | polyclonals |
| Alternative to gene target | SPHK, SPHK1 and IDBG-643068 and ENSBTAG00000008507 and 618605, SPHK1 and IDBG-69650 and ENSG00000176170 and 8877, Sphk1 and IDBG-214470 and ENSMUSG00000061878 and 20698, nuclei, protein phosphatase 2A binding, this GO :0000287 and magnesium ion binding and molecular function this GO :0001568 and blood vessel development and biological process this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0001956 and positive regulation of neurotransmitter secretion and biological process this GO :0003677 and DNA binding and molecular function this GO :0003951 and NAD+ kinase activity and molecular function this GO :0004143 and diacylglycerol kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005516 and calmodulin binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006457 and protein folding and biological process this GO :0006665 and sphin this GO lipid metabolic process and biological process this GO :0006670 and sphin this GO sine metabolic process and biological process this GO :0006954 and inflammatory response and biological process this GO :0007165 and signal transduction and biological process this GO :0007205 and protein kinase C-activating G-protein coupled receptor signaling pathway and biological process this GO :0007420 and brain development and biological process this GO :0007565 and female pregnancy and biological process this GO :0008021 and synaptic vesicle and cellular component this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008481 and sphinganine kinase activity and molecular function this GO :0009267 and cellular response to starvation and biological process this GO :0010803 and regulation of tumor necrosis factor-mediated signaling pathway and biological process this GO :0010976 and positive regulation of neuron projection development and biological process this GO :0014070 and response to organic cyclic compound and biological process this GO :0014075 and response to amine and biological process this GO :0016310 and phosphorylation and biological process this GO :0017050 and D-erythro-sphin this GO sine kinase activity and molecular function this GO :0019371 and cyclooxygenase pathway and biological process this GO :0019722 and calcium-mediated signaling and biological process this GO :0030148 and sphin this GO lipid biosynthetic process and biological process this GO :0030307 and positive regulation of cell growth and biological process this GO :0030335 and positive regulation of cell migration and biological process this GO :0030424 and axon and cellular component this GO :0031398 and positive regulation of protein ubiquitination and biological process this GO :0032026 and response to magnesium ion and biological process this GO :0032570 and response to progesterone and biological process this GO :0032651 and regulation of interleukin-1 beta production and biological process this GO :0033198 and response to ATP and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0042346 and positive regulation of NF-kappaB import into nucleus and biological process this GO :0043005 and neuron projection and cellular component this GO :0043066 and negative regulation of apoptotic process and biological process this GO :0044267 and cellular protein metabolic process and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045766 and positive regulation of angiogenesis and biological process this GO :0045931 and positive regulation of mitotic cell cycle and biological process this GO :0045987 and positive regulation of smooth muscle contraction and biological process this GO :0046521 and sphin this GO id catabolic process and biological process this GO :0046834 and lipid phosphorylation and biological process this GO :0048146 and positive regulation of fibroblast proliferation and biological process this GO :0051084 and 'de novo' posttranslational protein folding and biological process this GO :0051092 and positive regulation of NF-kappaB transcription factor activity and biological process this GO :0051721 and protein phosphatase 2A binding and molecular function this GO :0070301 and cellular response to hydrogen peroxide and biological process this GO :0070555 and response to interleukin-1 and biological process this GO :0071363 and cellular response to growth factor stimulus and biological process, this GO :0000287: magnesium ion binding, this GO :0000287: magnesium ion binding and also this GO :0003677: DNA binding and also this GO :0003951: NAD+ kinase activity and also this GO :0004143: diacylglycerol kinase activity and also this GO :0005515: protein binding and also this GO :0005516: calmodulin binding and also this GO :0005524: ATP binding and also this GO :0008481: sphinganine kinase activity and also this GO :0017050: D-erythro-sphin this GO sine kinase activity and also this GO :0051721: protein phosphatase 2A binding, this GO :0003677: DNA binding, this GO :0003951: NAD+ kinase activity, this GO :0004143: diacylglycerol kinase activity, this GO :0005515: protein binding, this GO :0005516: calmodulin binding, this GO :0005524: ATP binding, this GO :0008481: sphinganine kinase activity, this GO :0017050: D-erythro-sphin this GO sine kinase activity, this GO :0051721: protein phosphatase 2A binding, sphin this GO sine kinase 1 |
| Identity | 11240 |
| Gene | SPHK1 |
| Long gene name | sphingosine kinase 1 |
| Locus | 17q25, 2 |
| Discovery year | 1999-02-01 |
| GenBank acession | BC030553 |
| Entrez gene record | 8877 |
| Pubmed identfication | 9726979 |
| RefSeq identity | NM_182965 NM_021972 |
| Havana BLAST/BLAT | OTTHUMG00000180177 |