Anti-Src(Tyr215) (Polyclonal), ALEXA Fluor 594

Anti-Src(Tyr215) (Polyclonal), ALEXA Fluor 594 size: 100 microliters 516

Price 516

Size 100 microliters

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Type	Conjugated Primary Antibody
Conjugated with	594, ALEXA FLUOR®
Host organism	Rabbit (Oryctolagus cuniculus)
Target Protein/Peptide	Src Tyr215
Specificity	This antibody reacts specifically with Src(Tyr215)
Modification	Phosphorylation
Modification site	Tyr215
Clonality	Polyclonal Antibody
Clone	Polyclonal Antibodies
Concentration	1ug per 1ul
Subcellular locations	N/A
Antigen Source	KLH conjugated synthetic phosphopeptide derived from mouse Src around the phosphorylation site of Tyr215
Gene ID	6714
Swiss Prot	N/A
Applications	IF(IHC-P)
Applications with corresponding dilutions	IF(IHC-P)(1:50-200)
Cross reactive species	Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens)
Cross Reactive Species details	However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information	Full catalytic activity of Src requires phosphorylation of tyrosine 418, Immunogen: Synthetic peptide (Human) derived from the region of Src that contains tyrosine 529, Mutations in this gene could be involved in the malignant progression of colon cancer, Src is a proto oncogene that may play a role in the regulation of embryonic development and cell growth, The sequence is conserved in mouse (tyrosine 534), The tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites, The tyrosine 529 is located near the carboxyl terminus of Src and acts as a negative regulator, There are two critical phosphorylation sites of tyrosine on Src, This conformation blocks phosphorylation of tyrosine 418 at the catalytic domain, When tyrosine 529 is dephosphorylated, based on the human sequence, chicken (tyrosine 527) and frog (tyrosine 525), in that Src is held in the inactive form through an intramolecular interaction between the SH2 domain and the carboxyl terminus when tyrosine 529 is phosphorylated by Csk, thereby preventing Src activation, tyrosine 418 and tyrosine 529 (referring to human Src sequence), tyrosine 418 can be maximally phosphorylated and Src becomes active, Src (also known as pp60src) is a non receptor Tyrosine Kinase involved in signal transduction in many biological systems and implicated in the development of human tumors
Purification method	Purified by Protein A
Storage	50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission	590nm/617nm
Synonyms	ASV, Avian sarcoma virus, CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA, EC 2, Neuronal CSRC tyrosine specic protein kinase, Neuronal SRC, OTTHUMP00000030931, OTTHUMP00000174476, OTTHUMP00000174477, Oncogene SRC, Proto oncogene tyrosine protein kinase Src, Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, Protooncogene SRC, SRC Oncogene, SRC1, SRC_HUMAN, Src, Tyrosine kinase pp60c src, Tyrosine protein kinase SRC 1, Tyrosine protein kinase SRC1, V src sarcoma Schmidt Ruppin A 2 viral oncogene homolog avian, c SRC, cSrc, p60 Src, p60-Src, p60Src, pp60c src, pp60c-src, pp60csrc, protooncogene SRC Rous sarcoma, v src avian sarcoma Schmidt Ruppin A2 viral oncogene homolog, v src sarcoma Schmidt Ruppin A 2 viral oncogene homolog avian, vsrc avian sarcoma Schmidt Ruppin A2 viral oncogene homolog, 2, 7, 10, SRCphospho Y215
Also known as	Src(Tyr215) Polyclonal Antibody
Other name	Anti-Src(Tyr215) Polyclonal
Advisory	For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
Properties	For facs or microscopy Alexa 1 conjugate
Conjugation	Alexa Fluor
Group	Polyclonals and antibodies
About	The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene target	Src(Tyr215)
Short name	Anti-Src(Tyr215) (Polyclonal) Fluor 594
Technique	Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different
Label	ALEXA
Alternative name	ALEXA Fluor 594, antibody to-v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)(Tyr215) (polyclonal)
Alternative technique	polyclonals

Anti-Src(Tyr215) (Polyclonal), ALEXA Fluor 594

Anti-Src(Tyr215) (Polyclonal), ALEXA Fluor 594 size: 100 microliters 516

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