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Anti-UBE2E1 (Polyclonal), ALEXA Fluor 594

Anti-UBE2E1 (Polyclonal), ALEXA Fluor 594 size: 100 microliters 489

Supplier BIOSS POLYCLONAL ANTIBODIES
Price 489
Size 100 microliters
Contact us Ask Component cell markers and DNA bp. markers are genetic markerser genes or DNA sequenceswith selection audit
TypeConjugated Primary Antibody
Conjugated with 594, ALEXA FLUOR®
Host organismRabbit (Oryctolagus cuniculus)
Target Protein/PeptideUBE2E1
SpecificityThis antibody reacts specifically with UBE2E1
ModificationNo modification has been applied to this antibody
Modification siteNone
ClonalityPolyclonal Antibody
ClonePolyclonal Antibodies
Concentration1ug per 1ul
Subcellular locationsN/A
Antigen SourceKLH conjugated synthetic peptide derived from human UBCH6/UBE2E1
Gene ID7324
Swiss ProtN/A
ApplicationsIF(IHC-P)
Applications with corresponding dilutionsIF(IHC-P)(1:50-200)
Cross reactive species Rat (Rattus norvegicus), Mouse (Mus musculus)
Cross Reactive Species details However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species
Background information NF-kB maturation, Specifically, The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex, The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate, The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component, UBE2E1 and UBE2L3, UBE2E1 interacts with the tumor suppressor protein TSSC5, UBE2L3 has been shown to mediate c-fos degradation, also designated UBCH6 and UBCH7 respectively in human, are E2 conjugating enzymes that interact with various proteins, human papilloma virus-mediated p53 and Myc protein degradation, The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates
Purification methodPurified by Protein A
Storage 50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA
Excitation emission590nm/617nm
Synonyms UbcH6
Also known asUBE2E1 Polyclonal Antibody
Other nameAnti-UBE2E1 Polyclonal
Advisory For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody
PropertiesFor facs or microscopy Alexa 1 conjugate
ConjugationAlexa Fluor
GroupPolyclonals and antibodies
About The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot
Gene targetUBE2E1
Short nameAnti-UBE2E1 (Polyclonal) Fluor 594
Technique Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different 
LabelALEXA
Alternative name ALEXA Fluor 594, antibody to-UBE2E1 (polyclonal)
Alternative techniquepolyclonals
Identity 12477
Gene UBE2E1
Long gene name ubiquitin conjugating enzyme E2 E1
Synonyms gene name ubiquitin-conjugating enzyme E2E 1 (homologous to yeast UBC4/5) ubiquitin-conjugating enzyme E2E 1 (UBC4/5 homolog, yeast) ubiquitin-conjugating enzyme E2E 1
Locus 3p24, 2
Discovery year 1997-03-21
GenBank acession X92963
Entrez gene record 7324
Pubmed identfication 8576257
RefSeq identity NM_003341
Classification Ubiquitin conjugating enzymes E2
Havana BLAST/BLAT OTTHUMG00000130483

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